Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens

Kazuhiko Nakano, Hiroaki Inaba, Ryota Nomura, Hirotoshi Nemoto, Munehiro Takeda, Hideo Yoshioka, Hajime Matsue, Toshiki Takahashi, Kazuhiro Taniguchi, Atsuo Amano, Takashi Ooshima

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

The involvement of oral bacteria in the pathogenesis of cardiovascular diseases has been the focus of attention in many studies, and several periodontal pathogens have been detected in diseased cardiovascular lesions, suggesting relationships between oral microorganisms and cardiovascular diseases. However, no information is available regarding the involvement of cariogenic pathogens such as Streptococcus mutans. The presence of oral streptococcal species and periodontitis-related bacteria in 35 heart valve and 27 atheromatous plaque clinical specimens, as well as 32 dental plaque specimens from the same subjects, was analyzed using a PCR method. Furthermore, broad-range PCR with DNA sequencing analysis was employed to identify the bacterial species in those samples. Streptococcus mutans was frequently detected in the heart valve (69%) and atheromatous plaque (74%) specimens, while other bacterial species, including those related to periodontitis, were detected with much lower frequencies. The bacterial composition in cardiovascular tissues was found to be markedly distinct from that in dental plaque, with only a limited number of species, including S. mutans, in the cardiovascular regions shown to have possibly originated from the oral cavity. Semiquantitative assay results revealed that S. mutans was detected in significant quantities in the heart valve (40%) and atheromatous plaque (48%) specimens, whereas the quantities of all other tested bacterial species, including several related to periodontitis, were negligible in the cardiovascular samples. These results indicate that S. mutans is a possible causative agent of cardiovascular disease.

Original languageEnglish
Pages (from-to)3313-3317
Number of pages5
JournalJournal of Clinical Microbiology
Volume44
Issue number9
DOIs
Publication statusPublished - Sep 2006
Externally publishedYes

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Streptococcus mutans
Heart Valves
Atherosclerotic Plaques
Periodontitis
Cardiovascular Diseases
Dental Plaque
Mouth Diseases
Bacteria
Polymerase Chain Reaction
DNA Sequence Analysis
Mouth

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens. / Nakano, Kazuhiko; Inaba, Hiroaki; Nomura, Ryota; Nemoto, Hirotoshi; Takeda, Munehiro; Yoshioka, Hideo; Matsue, Hajime; Takahashi, Toshiki; Taniguchi, Kazuhiro; Amano, Atsuo; Ooshima, Takashi.

In: Journal of Clinical Microbiology, Vol. 44, No. 9, 09.2006, p. 3313-3317.

Research output: Contribution to journalArticle

Nakano, K, Inaba, H, Nomura, R, Nemoto, H, Takeda, M, Yoshioka, H, Matsue, H, Takahashi, T, Taniguchi, K, Amano, A & Ooshima, T 2006, 'Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens', Journal of Clinical Microbiology, vol. 44, no. 9, pp. 3313-3317. https://doi.org/10.1128/JCM.00377-06
Nakano, Kazuhiko ; Inaba, Hiroaki ; Nomura, Ryota ; Nemoto, Hirotoshi ; Takeda, Munehiro ; Yoshioka, Hideo ; Matsue, Hajime ; Takahashi, Toshiki ; Taniguchi, Kazuhiro ; Amano, Atsuo ; Ooshima, Takashi. / Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens. In: Journal of Clinical Microbiology. 2006 ; Vol. 44, No. 9. pp. 3313-3317.
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