Detection of bcl-2 protein and bcl-2 messenger RNA in normal and neoplastic lymphoid tissues by immunohistochemistry and in situ hybridization

Eisaku Kondo, Shuji Nakamura, Hitoshi Onoue, Yoshinobu Matsuo, Tadashi Yoshino, Hiroyuki Aoki, Kazuhiko Hayashi, Kiyoshi Takahashi, Jun Minowada, Shintaro Nomura, Tadaatsu Akagi

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Abstract

bcl-2 protein has been detected in surgical specimens and cultured permanent cell lines of non-Hodgkin's lymphomas and leukemias using enzyme immunohistochemistry and immunofluorescence with anti-bcl-2 monoclonal antibodies. Of 40 surgical specimens, bcl-2 protein was expressed in 50% of B-cell and 41% of T-cell lymphomas, both with and without the bcl-2 gene rearrangement. In investigations of 38 hematopoietic cell lines, bcl-2 protein was detected not only in lymphoid cell lines but also in myeloid cell lines. In situ hybridization and immunohistochemical analysis of reactive lymph nodes showed that lymphocytes in mantle zones and paracortical areas expressed bcl-2 protein consistent with the messenger RNA distribution and that germinal center cells showed abundant bcl-2 transcript, despite the absence of detectable bcl-2 protein. These results suggest that bcl-2 protein is broadly expressed in various hematopoietic neoplasms not restricted in t(14;18) lymphomas and that germinal center cells may be involved in some arrest of bcl-2 protein expression at the posttranscriptional level.

Original languageEnglish
Pages (from-to)2044-2051
Number of pages8
JournalBlood
Volume80
Issue number8
Publication statusPublished - Oct 15 1992

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Lymphoid Tissue
In Situ Hybridization
Immunohistochemistry
Tissue
Messenger RNA
Cells
Proteins
Cell Line
Germinal Center
Lymphocytes
bcl-2 Genes
T-cells
Gene Rearrangement
T-Cell Lymphoma
Hematologic Neoplasms
Myeloid Cells
Non-Hodgkin's Lymphoma
Fluorescent Antibody Technique
Cultured Cells
Lymphoma

ASJC Scopus subject areas

  • Hematology

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Detection of bcl-2 protein and bcl-2 messenger RNA in normal and neoplastic lymphoid tissues by immunohistochemistry and in situ hybridization. / Kondo, Eisaku; Nakamura, Shuji; Onoue, Hitoshi; Matsuo, Yoshinobu; Yoshino, Tadashi; Aoki, Hiroyuki; Hayashi, Kazuhiko; Takahashi, Kiyoshi; Minowada, Jun; Nomura, Shintaro; Akagi, Tadaatsu.

In: Blood, Vol. 80, No. 8, 15.10.1992, p. 2044-2051.

Research output: Contribution to journalArticle

Kondo, E, Nakamura, S, Onoue, H, Matsuo, Y, Yoshino, T, Aoki, H, Hayashi, K, Takahashi, K, Minowada, J, Nomura, S & Akagi, T 1992, 'Detection of bcl-2 protein and bcl-2 messenger RNA in normal and neoplastic lymphoid tissues by immunohistochemistry and in situ hybridization', Blood, vol. 80, no. 8, pp. 2044-2051.
Kondo, Eisaku ; Nakamura, Shuji ; Onoue, Hitoshi ; Matsuo, Yoshinobu ; Yoshino, Tadashi ; Aoki, Hiroyuki ; Hayashi, Kazuhiko ; Takahashi, Kiyoshi ; Minowada, Jun ; Nomura, Shintaro ; Akagi, Tadaatsu. / Detection of bcl-2 protein and bcl-2 messenger RNA in normal and neoplastic lymphoid tissues by immunohistochemistry and in situ hybridization. In: Blood. 1992 ; Vol. 80, No. 8. pp. 2044-2051.
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AU - Kondo, Eisaku

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AU - Matsuo, Yoshinobu

AU - Yoshino, Tadashi

AU - Aoki, Hiroyuki

AU - Hayashi, Kazuhiko

AU - Takahashi, Kiyoshi

AU - Minowada, Jun

AU - Nomura, Shintaro

AU - Akagi, Tadaatsu

PY - 1992/10/15

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N2 - bcl-2 protein has been detected in surgical specimens and cultured permanent cell lines of non-Hodgkin's lymphomas and leukemias using enzyme immunohistochemistry and immunofluorescence with anti-bcl-2 monoclonal antibodies. Of 40 surgical specimens, bcl-2 protein was expressed in 50% of B-cell and 41% of T-cell lymphomas, both with and without the bcl-2 gene rearrangement. In investigations of 38 hematopoietic cell lines, bcl-2 protein was detected not only in lymphoid cell lines but also in myeloid cell lines. In situ hybridization and immunohistochemical analysis of reactive lymph nodes showed that lymphocytes in mantle zones and paracortical areas expressed bcl-2 protein consistent with the messenger RNA distribution and that germinal center cells showed abundant bcl-2 transcript, despite the absence of detectable bcl-2 protein. These results suggest that bcl-2 protein is broadly expressed in various hematopoietic neoplasms not restricted in t(14;18) lymphomas and that germinal center cells may be involved in some arrest of bcl-2 protein expression at the posttranscriptional level.

AB - bcl-2 protein has been detected in surgical specimens and cultured permanent cell lines of non-Hodgkin's lymphomas and leukemias using enzyme immunohistochemistry and immunofluorescence with anti-bcl-2 monoclonal antibodies. Of 40 surgical specimens, bcl-2 protein was expressed in 50% of B-cell and 41% of T-cell lymphomas, both with and without the bcl-2 gene rearrangement. In investigations of 38 hematopoietic cell lines, bcl-2 protein was detected not only in lymphoid cell lines but also in myeloid cell lines. In situ hybridization and immunohistochemical analysis of reactive lymph nodes showed that lymphocytes in mantle zones and paracortical areas expressed bcl-2 protein consistent with the messenger RNA distribution and that germinal center cells showed abundant bcl-2 transcript, despite the absence of detectable bcl-2 protein. These results suggest that bcl-2 protein is broadly expressed in various hematopoietic neoplasms not restricted in t(14;18) lymphomas and that germinal center cells may be involved in some arrest of bcl-2 protein expression at the posttranscriptional level.

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