Detection of AP12-MALT1 chimaeric gene in extranodal and nodal marginal zone B-cell lymphoma by reverse transcription polymerase chain reaction (PCR) and genomic long and accurate PCR analyses

Masakatsu Yonezumi, Ritsuro Suzuki, Hiroko Suzuki, Tadashi Yoshino, Kouichi Oshima, Yoshitaka Hosokawa, Masahiro Asaka, Yasuo Morishima, Shigeo Nakamura, Masao Seto

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

t(11;18)(q21;q21) has been recognized as a characteristic chromosomal translocation in mucosa-associated lymphoid tissue (MALT)-type lymphoma, and recent studies have demonstrated that this translocation results in the chimaeric transcript of API2 (apoptosis inhibitor 2)-MALT1 (mucosa-associated lymphoid tissue lymphoma translocation gene 1). In this study, we used reverse transcription polymerase chain reaction (RT-PCR) to analyse the incidence of this fusion product in a large series of MALT lymphoma, nodal marginal zone B-cell lymphoma (nMZBCL) and extranodal diffuse large B-cell lymphoma (DLBL) cases. RT-PCR analysis revealed that 17 of the 95 (17.9%) MALT lymphomas but none of the nine nMZBCLs or 16 DLBLs had API2-MALT1 fusion transcripts. The incidence of API2-MALT1 varied among MALT lymphomas arising from different sites and was highest for pulmonary MALT lymphomas (10 out of 16 cases, 62.5%). The presence of the API2-MALT1 fusion gene was also confirmed by long and accurate (LA)-PCR with genomic DNA, and the result correlated well with that obtained with the RT-PCR assay, thus demonstrating the usefulness of LA-PCR for the detection of the API2-MALT1 fusion gene.

Original languageEnglish
Pages (from-to)588-594
Number of pages7
JournalBritish Journal of Haematology
Volume115
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Marginal Zone B-Cell Lymphoma
Reverse Transcription
Polymerase Chain Reaction
Apoptosis
Genes
Gene Fusion
Genetic Translocation
Lymphoma, Large B-Cell, Diffuse
Incidence
Lung
DNA

Keywords

  • API2
  • Chimaeric gene
  • Long and accurate polymerase chain reaction
  • Lymphoma
  • MALT1

ASJC Scopus subject areas

  • Hematology

Cite this

Detection of AP12-MALT1 chimaeric gene in extranodal and nodal marginal zone B-cell lymphoma by reverse transcription polymerase chain reaction (PCR) and genomic long and accurate PCR analyses. / Yonezumi, Masakatsu; Suzuki, Ritsuro; Suzuki, Hiroko; Yoshino, Tadashi; Oshima, Kouichi; Hosokawa, Yoshitaka; Asaka, Masahiro; Morishima, Yasuo; Nakamura, Shigeo; Seto, Masao.

In: British Journal of Haematology, Vol. 115, No. 3, 2001, p. 588-594.

Research output: Contribution to journalArticle

Yonezumi, Masakatsu ; Suzuki, Ritsuro ; Suzuki, Hiroko ; Yoshino, Tadashi ; Oshima, Kouichi ; Hosokawa, Yoshitaka ; Asaka, Masahiro ; Morishima, Yasuo ; Nakamura, Shigeo ; Seto, Masao. / Detection of AP12-MALT1 chimaeric gene in extranodal and nodal marginal zone B-cell lymphoma by reverse transcription polymerase chain reaction (PCR) and genomic long and accurate PCR analyses. In: British Journal of Haematology. 2001 ; Vol. 115, No. 3. pp. 588-594.
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abstract = "t(11;18)(q21;q21) has been recognized as a characteristic chromosomal translocation in mucosa-associated lymphoid tissue (MALT)-type lymphoma, and recent studies have demonstrated that this translocation results in the chimaeric transcript of API2 (apoptosis inhibitor 2)-MALT1 (mucosa-associated lymphoid tissue lymphoma translocation gene 1). In this study, we used reverse transcription polymerase chain reaction (RT-PCR) to analyse the incidence of this fusion product in a large series of MALT lymphoma, nodal marginal zone B-cell lymphoma (nMZBCL) and extranodal diffuse large B-cell lymphoma (DLBL) cases. RT-PCR analysis revealed that 17 of the 95 (17.9{\%}) MALT lymphomas but none of the nine nMZBCLs or 16 DLBLs had API2-MALT1 fusion transcripts. The incidence of API2-MALT1 varied among MALT lymphomas arising from different sites and was highest for pulmonary MALT lymphomas (10 out of 16 cases, 62.5{\%}). The presence of the API2-MALT1 fusion gene was also confirmed by long and accurate (LA)-PCR with genomic DNA, and the result correlated well with that obtained with the RT-PCR assay, thus demonstrating the usefulness of LA-PCR for the detection of the API2-MALT1 fusion gene.",
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AU - Suzuki, Ritsuro

AU - Suzuki, Hiroko

AU - Yoshino, Tadashi

AU - Oshima, Kouichi

AU - Hosokawa, Yoshitaka

AU - Asaka, Masahiro

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AU - Nakamura, Shigeo

AU - Seto, Masao

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AB - t(11;18)(q21;q21) has been recognized as a characteristic chromosomal translocation in mucosa-associated lymphoid tissue (MALT)-type lymphoma, and recent studies have demonstrated that this translocation results in the chimaeric transcript of API2 (apoptosis inhibitor 2)-MALT1 (mucosa-associated lymphoid tissue lymphoma translocation gene 1). In this study, we used reverse transcription polymerase chain reaction (RT-PCR) to analyse the incidence of this fusion product in a large series of MALT lymphoma, nodal marginal zone B-cell lymphoma (nMZBCL) and extranodal diffuse large B-cell lymphoma (DLBL) cases. RT-PCR analysis revealed that 17 of the 95 (17.9%) MALT lymphomas but none of the nine nMZBCLs or 16 DLBLs had API2-MALT1 fusion transcripts. The incidence of API2-MALT1 varied among MALT lymphomas arising from different sites and was highest for pulmonary MALT lymphomas (10 out of 16 cases, 62.5%). The presence of the API2-MALT1 fusion gene was also confirmed by long and accurate (LA)-PCR with genomic DNA, and the result correlated well with that obtained with the RT-PCR assay, thus demonstrating the usefulness of LA-PCR for the detection of the API2-MALT1 fusion gene.

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