Abstract
t(11;18)(q21;q21) has been recognized as a characteristic chromosomal translocation in mucosa-associated lymphoid tissue (MALT)-type lymphoma, and recent studies have demonstrated that this translocation results in the chimaeric transcript of API2 (apoptosis inhibitor 2)-MALT1 (mucosa-associated lymphoid tissue lymphoma translocation gene 1). In this study, we used reverse transcription polymerase chain reaction (RT-PCR) to analyse the incidence of this fusion product in a large series of MALT lymphoma, nodal marginal zone B-cell lymphoma (nMZBCL) and extranodal diffuse large B-cell lymphoma (DLBL) cases. RT-PCR analysis revealed that 17 of the 95 (17.9%) MALT lymphomas but none of the nine nMZBCLs or 16 DLBLs had API2-MALT1 fusion transcripts. The incidence of API2-MALT1 varied among MALT lymphomas arising from different sites and was highest for pulmonary MALT lymphomas (10 out of 16 cases, 62.5%). The presence of the API2-MALT1 fusion gene was also confirmed by long and accurate (LA)-PCR with genomic DNA, and the result correlated well with that obtained with the RT-PCR assay, thus demonstrating the usefulness of LA-PCR for the detection of the API2-MALT1 fusion gene.
| Original language | English |
|---|---|
| Pages (from-to) | 588-594 |
| Number of pages | 7 |
| Journal | British Journal of Haematology |
| Volume | 115 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- API2
- Chimaeric gene
- Long and accurate polymerase chain reaction
- Lymphoma
- MALT1
ASJC Scopus subject areas
- Hematology
Cite this
Detection of AP12-MALT1 chimaeric gene in extranodal and nodal marginal zone B-cell lymphoma by reverse transcription polymerase chain reaction (PCR) and genomic long and accurate PCR analyses. / Yonezumi, Masakatsu; Suzuki, Ritsuro; Suzuki, Hiroko; Yoshino, Tadashi; Oshima, Kouichi; Hosokawa, Yoshitaka; Asaka, Masahiro; Morishima, Yasuo; Nakamura, Shigeo; Seto, Masao.
In: British Journal of Haematology, Vol. 115, No. 3, 2001, p. 588-594.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of AP12-MALT1 chimaeric gene in extranodal and nodal marginal zone B-cell lymphoma by reverse transcription polymerase chain reaction (PCR) and genomic long and accurate PCR analyses
AU - Yonezumi, Masakatsu
AU - Suzuki, Ritsuro
AU - Suzuki, Hiroko
AU - Yoshino, Tadashi
AU - Oshima, Kouichi
AU - Hosokawa, Yoshitaka
AU - Asaka, Masahiro
AU - Morishima, Yasuo
AU - Nakamura, Shigeo
AU - Seto, Masao
PY - 2001
Y1 - 2001
N2 - t(11;18)(q21;q21) has been recognized as a characteristic chromosomal translocation in mucosa-associated lymphoid tissue (MALT)-type lymphoma, and recent studies have demonstrated that this translocation results in the chimaeric transcript of API2 (apoptosis inhibitor 2)-MALT1 (mucosa-associated lymphoid tissue lymphoma translocation gene 1). In this study, we used reverse transcription polymerase chain reaction (RT-PCR) to analyse the incidence of this fusion product in a large series of MALT lymphoma, nodal marginal zone B-cell lymphoma (nMZBCL) and extranodal diffuse large B-cell lymphoma (DLBL) cases. RT-PCR analysis revealed that 17 of the 95 (17.9%) MALT lymphomas but none of the nine nMZBCLs or 16 DLBLs had API2-MALT1 fusion transcripts. The incidence of API2-MALT1 varied among MALT lymphomas arising from different sites and was highest for pulmonary MALT lymphomas (10 out of 16 cases, 62.5%). The presence of the API2-MALT1 fusion gene was also confirmed by long and accurate (LA)-PCR with genomic DNA, and the result correlated well with that obtained with the RT-PCR assay, thus demonstrating the usefulness of LA-PCR for the detection of the API2-MALT1 fusion gene.
AB - t(11;18)(q21;q21) has been recognized as a characteristic chromosomal translocation in mucosa-associated lymphoid tissue (MALT)-type lymphoma, and recent studies have demonstrated that this translocation results in the chimaeric transcript of API2 (apoptosis inhibitor 2)-MALT1 (mucosa-associated lymphoid tissue lymphoma translocation gene 1). In this study, we used reverse transcription polymerase chain reaction (RT-PCR) to analyse the incidence of this fusion product in a large series of MALT lymphoma, nodal marginal zone B-cell lymphoma (nMZBCL) and extranodal diffuse large B-cell lymphoma (DLBL) cases. RT-PCR analysis revealed that 17 of the 95 (17.9%) MALT lymphomas but none of the nine nMZBCLs or 16 DLBLs had API2-MALT1 fusion transcripts. The incidence of API2-MALT1 varied among MALT lymphomas arising from different sites and was highest for pulmonary MALT lymphomas (10 out of 16 cases, 62.5%). The presence of the API2-MALT1 fusion gene was also confirmed by long and accurate (LA)-PCR with genomic DNA, and the result correlated well with that obtained with the RT-PCR assay, thus demonstrating the usefulness of LA-PCR for the detection of the API2-MALT1 fusion gene.
KW - API2
KW - Chimaeric gene
KW - Long and accurate polymerase chain reaction
KW - Lymphoma
KW - MALT1
UR - http://www.scopus.com/inward/record.url?scp=0035672788&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035672788&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2141.2001.03158.x
DO - 10.1046/j.1365-2141.2001.03158.x
M3 - Article
C2 - 11736940
AN - SCOPUS:0035672788
VL - 115
SP - 588
EP - 594
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 3
ER -