Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus

Ayano Satoh, Kimihiro Suzuki, Eiji Takayama, Kyoko Kojima, Toshihiko Hidaka, Makoto Kawakami, Isamu Matsumoto, Fumitaka Ohsuzu

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Objective. Annexins (Anx) are a family of structurally related proteins that bind to phospholipids in a calcium dependent manner. It has been reported that antibodies to Anx V, which acts as an antithrombotic protein, are associated with thrombosis in systemic lupus erythematosus (SLE) and/or antiphospholipid syndrome (APS). Homology between the primary structures of Anx IV and Anx V is the highest among members of the Anx family. We investigated whether anti-Anx IV autoantibodies can be detected in the sera of patients with SLE and/or APS. Methods. Seventy-four patients with SLE/APS were divided into 3 groups: Group A, patients with SLE but no clinical or serological features of APS; Group B, patients with SLE having only serological signs of APS; and Group C, patients with clinical symptoms and serological signs of APS. Anx IV and Anx V were prepared by recombinant technique. Anti-Anx IV, Anx V, cardiolipin (CL), and CL β2-glycoprotein I were detected by ELISA. Results. Anti-Anx IV was found in 15.4% of Group A, 20.0% of Group B, and 21.7% of Group C. Anti-Anx V was found in 3.8% of Group A, 28.0% of Group B, and 30.4% of Group C. Significant correlations were noted between anti-Anx IV titer and anti-Anx V titer (p <0.001), and between anti-Anx IV titer and aCL titer (p <0.01). Conclusion. Anti-Anx IV and V antibodies were characterized in the sera of patients with SLE/APS. Significantly higher frequency of arterial or venous thrombosis was found in patients with anti-Anx V.

Original languageEnglish
Pages (from-to)1715-1720
Number of pages6
JournalJournal of Rheumatology
Volume26
Issue number8
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Annexin A4
Antiphospholipid Syndrome
Annexin A5
Systemic Lupus Erythematosus
Antibodies
Annexins
Cardiolipins
Serum
Venous Thrombosis
Autoantibodies
Signs and Symptoms
Phospholipids
Glycoproteins
Proteins
Thrombosis
Enzyme-Linked Immunosorbent Assay

Keywords

  • Annexins
  • Antiphospholipid syndrome
  • Systemic lupus erythematosus

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Satoh, A., Suzuki, K., Takayama, E., Kojima, K., Hidaka, T., Kawakami, M., ... Ohsuzu, F. (1999). Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus. Journal of Rheumatology, 26(8), 1715-1720.

Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus. / Satoh, Ayano; Suzuki, Kimihiro; Takayama, Eiji; Kojima, Kyoko; Hidaka, Toshihiko; Kawakami, Makoto; Matsumoto, Isamu; Ohsuzu, Fumitaka.

In: Journal of Rheumatology, Vol. 26, No. 8, 1999, p. 1715-1720.

Research output: Contribution to journalArticle

Satoh, A, Suzuki, K, Takayama, E, Kojima, K, Hidaka, T, Kawakami, M, Matsumoto, I & Ohsuzu, F 1999, 'Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus', Journal of Rheumatology, vol. 26, no. 8, pp. 1715-1720.
Satoh, Ayano ; Suzuki, Kimihiro ; Takayama, Eiji ; Kojima, Kyoko ; Hidaka, Toshihiko ; Kawakami, Makoto ; Matsumoto, Isamu ; Ohsuzu, Fumitaka. / Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus. In: Journal of Rheumatology. 1999 ; Vol. 26, No. 8. pp. 1715-1720.
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title = "Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus",
abstract = "Objective. Annexins (Anx) are a family of structurally related proteins that bind to phospholipids in a calcium dependent manner. It has been reported that antibodies to Anx V, which acts as an antithrombotic protein, are associated with thrombosis in systemic lupus erythematosus (SLE) and/or antiphospholipid syndrome (APS). Homology between the primary structures of Anx IV and Anx V is the highest among members of the Anx family. We investigated whether anti-Anx IV autoantibodies can be detected in the sera of patients with SLE and/or APS. Methods. Seventy-four patients with SLE/APS were divided into 3 groups: Group A, patients with SLE but no clinical or serological features of APS; Group B, patients with SLE having only serological signs of APS; and Group C, patients with clinical symptoms and serological signs of APS. Anx IV and Anx V were prepared by recombinant technique. Anti-Anx IV, Anx V, cardiolipin (CL), and CL β2-glycoprotein I were detected by ELISA. Results. Anti-Anx IV was found in 15.4{\%} of Group A, 20.0{\%} of Group B, and 21.7{\%} of Group C. Anti-Anx V was found in 3.8{\%} of Group A, 28.0{\%} of Group B, and 30.4{\%} of Group C. Significant correlations were noted between anti-Anx IV titer and anti-Anx V titer (p <0.001), and between anti-Anx IV titer and aCL titer (p <0.01). Conclusion. Anti-Anx IV and V antibodies were characterized in the sera of patients with SLE/APS. Significantly higher frequency of arterial or venous thrombosis was found in patients with anti-Anx V.",
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T1 - Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus

AU - Satoh, Ayano

AU - Suzuki, Kimihiro

AU - Takayama, Eiji

AU - Kojima, Kyoko

AU - Hidaka, Toshihiko

AU - Kawakami, Makoto

AU - Matsumoto, Isamu

AU - Ohsuzu, Fumitaka

PY - 1999

Y1 - 1999

N2 - Objective. Annexins (Anx) are a family of structurally related proteins that bind to phospholipids in a calcium dependent manner. It has been reported that antibodies to Anx V, which acts as an antithrombotic protein, are associated with thrombosis in systemic lupus erythematosus (SLE) and/or antiphospholipid syndrome (APS). Homology between the primary structures of Anx IV and Anx V is the highest among members of the Anx family. We investigated whether anti-Anx IV autoantibodies can be detected in the sera of patients with SLE and/or APS. Methods. Seventy-four patients with SLE/APS were divided into 3 groups: Group A, patients with SLE but no clinical or serological features of APS; Group B, patients with SLE having only serological signs of APS; and Group C, patients with clinical symptoms and serological signs of APS. Anx IV and Anx V were prepared by recombinant technique. Anti-Anx IV, Anx V, cardiolipin (CL), and CL β2-glycoprotein I were detected by ELISA. Results. Anti-Anx IV was found in 15.4% of Group A, 20.0% of Group B, and 21.7% of Group C. Anti-Anx V was found in 3.8% of Group A, 28.0% of Group B, and 30.4% of Group C. Significant correlations were noted between anti-Anx IV titer and anti-Anx V titer (p <0.001), and between anti-Anx IV titer and aCL titer (p <0.01). Conclusion. Anti-Anx IV and V antibodies were characterized in the sera of patients with SLE/APS. Significantly higher frequency of arterial or venous thrombosis was found in patients with anti-Anx V.

AB - Objective. Annexins (Anx) are a family of structurally related proteins that bind to phospholipids in a calcium dependent manner. It has been reported that antibodies to Anx V, which acts as an antithrombotic protein, are associated with thrombosis in systemic lupus erythematosus (SLE) and/or antiphospholipid syndrome (APS). Homology between the primary structures of Anx IV and Anx V is the highest among members of the Anx family. We investigated whether anti-Anx IV autoantibodies can be detected in the sera of patients with SLE and/or APS. Methods. Seventy-four patients with SLE/APS were divided into 3 groups: Group A, patients with SLE but no clinical or serological features of APS; Group B, patients with SLE having only serological signs of APS; and Group C, patients with clinical symptoms and serological signs of APS. Anx IV and Anx V were prepared by recombinant technique. Anti-Anx IV, Anx V, cardiolipin (CL), and CL β2-glycoprotein I were detected by ELISA. Results. Anti-Anx IV was found in 15.4% of Group A, 20.0% of Group B, and 21.7% of Group C. Anti-Anx V was found in 3.8% of Group A, 28.0% of Group B, and 30.4% of Group C. Significant correlations were noted between anti-Anx IV titer and anti-Anx V titer (p <0.001), and between anti-Anx IV titer and aCL titer (p <0.01). Conclusion. Anti-Anx IV and V antibodies were characterized in the sera of patients with SLE/APS. Significantly higher frequency of arterial or venous thrombosis was found in patients with anti-Anx V.

KW - Annexins

KW - Antiphospholipid syndrome

KW - Systemic lupus erythematosus

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