Detection of a quantitative trait locus controlling carbon isotope discrimination and its contribution to stomatal conductance in japonica rice

Toshiyuki Takai, Akihiro Ohsumi, Yumiko San-Oh, Ma Rebecca C. Laza, Motohiko Kondo, Toshio Yamamoto, Masahiro Yano

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Increasing leaf photosynthesis offers a possible way to improve yield potential in rice (Oryza sativa L.). Carbon isotope discrimination (Δ13C) has potential as an indirect selection criterion. In this study, we searched for quantitative trait loci (QTLs) controlling Δ13C, and assessed their association with leaf photosynthesis. Substitution mapping by using chromosome segment substitution lines (CSSLs), that carry segments from the indica cultivar Kasalath in the genetic background of the japonica cultivar Koshihikari, identified genomic regions affecting Δ13C on chromosomes (Chr.) 2, 3, 6, 7, and 12. One of the CSSLs, SL208, in which most regions on Chr. 3 were substituted with Kasalath segments, showed higher leaf stomatal conductance for CO2 (g s) and Δ13C than Koshihikari during the vegetative stage although leaf photosynthetic rate did not differ between them. These results suggest an association between Δ13C and g s. To test this association, we performed a QTL analysis for Δ13C at vegetative and heading stages in an F2 population derived from a cross between SL208 and Koshihikari. The results confirmed a QTL controlling Δ13C on the long arm of Chr. 3. By using a near-isogenic line specific to Hd6, we ruled out the possibility that variation in Δ13C was generated through the pleiotropic effect of heading date.

Original languageEnglish
Pages (from-to)1401-1410
Number of pages10
JournalTheoretical and Applied Genetics
Volume118
Issue number7
DOIs
Publication statusPublished - May 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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