Detection of a novel locus involved in non-seed-shattering behaviour of Japonica rice cultivar, Oryza sativa ‘Nipponbare’

Yuki Tsujimura, Shohei Sugiyama, Kotaro Otsuka, Than Myint Htun, Koji Numaguchi, Cristina Castillo, Takashi Akagi, Takashige Ishii, Ryo Ishikawa

Research output: Contribution to journalArticle

Abstract

Key message: A novel locus, qCSS3, involved in the non-seed-shattering behaviour of Japonica rice cultivar, ‘Nipponbare’, was detected by QTL-seq analysis using the segregating population with the fixed known seed-shattering loci. Abstract: Asian cultivated rice, Oryzasativa, was domesticated from its wild ancestor, O.rufipogon. Loss of seed shattering is one of the most recognisable traits selected during rice domestication. Three quantitative trait loci (QTLs), qSH1, qSH3, and sh4, were previously reported to be involved in the loss of seed shattering of Japonica cultivated rice, O.sativa ‘Nipponbare’.However, the introgression line (IL) carrying ‘Nipponbare’ alleles at these three loci in the genetic background of wild rice, O.rufipogon W630, showed a lower value for detaching a grain from the pedicel than ‘Nipponbare’. Here, we investigated abscission layer formation in the IL and found a partially formed abscission layer in the central region between the epidermis and vascular bundles. Based on QTL-seq analysis using the F2 population obtained from a cross between ‘Nipponbare’ and the IL, we detected two novel loci qCSS3 and qCSS9 (QTL for the Control of Seed Shattering in rice on chromosomes 3 and 9), which were found to be involved in the difference in seed-shattering degree between ‘Nipponbare’ and W630. Then, we further focused on qCSS3 in order to understand its potential role on the loss of seed shattering. The candidate region of qCSS3 was found to be located within a 526-kb region using substitution mapping analysis. Interestingly, the qCSS3 candidate region partially overlaps the selective sweep detected for Japonica but not for Indica rice cultivars, suggesting that this region harbours the mutation at a novel seed-shattering locus specifically selected for non-seed-shattering behaviour in Japonica cultivars.

Original languageEnglish
Pages (from-to)2615-2623
Number of pages9
JournalTheoretical and Applied Genetics
Volume132
Issue number9
DOIs
Publication statusPublished - Sep 1 2019

    Fingerprint

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Tsujimura, Y., Sugiyama, S., Otsuka, K., Htun, T. M., Numaguchi, K., Castillo, C., Akagi, T., Ishii, T., & Ishikawa, R. (2019). Detection of a novel locus involved in non-seed-shattering behaviour of Japonica rice cultivar, Oryza sativa ‘Nipponbare’. Theoretical and Applied Genetics, 132(9), 2615-2623. https://doi.org/10.1007/s00122-019-03376-3