Detection of a heat stress-mediated interaction between protein and phospholipid membrane using dielectric measurement

Seiichi Morita, Toshinori Shimanouchi, Masashi Sasaki, Hiroshi Umakoshi, Ryoichi Kuboi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The dielectric response of lipid bilayer membrane vesicles (liposomes) prepared using either phosphatidylcholine from egg (EPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was analyzed at a frequency range of 0.1 to 100 MHz. A marked dielectric dispersion for EPC and POPC liposome suspensions was observed above 1 MHz. An appropriate analysis of the dielectric dispersion curve was performed using the Cole-Cole equation and the Debye equation and was found to provide a method for the determination of dielectric parameters. Among the dielectric parameters, the characteristic frequency of a second dispersion around 50 MHz varied corresponding with changes in the test conditions. Of particular note is that an anomalous change in the characteristic frequency in the presence of protein corresponded to the degree of hydrophobic interaction between proteins and liposomes. The value of the frequency around 50 MHz, as well as the decrease in permittivity over the frequency range tested, are indicators of the interaction between proteins and liposomes.

Original languageEnglish
Pages (from-to)252-256
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume95
Issue number3
DOIs
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

Liposomes
Phospholipids
Membrane Proteins
Hot Temperature
Proteins
Membranes
Permittivity
Lipid bilayers
Phosphorylcholine
Lipid Bilayers
Membrane Lipids
Phosphatidylcholines
Hydrophobic and Hydrophilic Interactions
Ovum
Suspensions
erucylphosphocholine

Keywords

  • Biosensor
  • Dielectric dispersion
  • Lipid bilayer
  • Liposome
  • Protein-phospholipid membrane interaction
  • Stress response

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Detection of a heat stress-mediated interaction between protein and phospholipid membrane using dielectric measurement. / Morita, Seiichi; Shimanouchi, Toshinori; Sasaki, Masashi; Umakoshi, Hiroshi; Kuboi, Ryoichi.

In: Journal of Bioscience and Bioengineering, Vol. 95, No. 3, 2003, p. 252-256.

Research output: Contribution to journalArticle

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AU - Kuboi, Ryoichi

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