Detection of a 4DL chromosome segment translocated to rye chromosome 5R in an advanced hexaploid triticale line Bronco 90

Shin Taketa, Tetsuya Nakazaki, Trude Schwarzacher, John S. Heslop-Harrison

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bronco 90 is an advanced line of hexaploid triticale and was reported to be a 2D(2R) chromosome substitution type. In F1 hybrids of this triticale with bread wheat, however, a meiotic configuration of 16 bivalents and 10 univalents was frequently observed indicating the presence of an additional D(R) chromosome substitution or D/R translocation. To determine the chromosome constitution of Bronco 90, C-banding and fluorescent in sire hybridization techniques were applied to somatic and meiotic metaphase chromosomes. These analyses revealed that in Bronco 90, the terminal 7% of the long arm of rye chromosome 5R is derived from the long arm of chromosome 4D. This translocated chromosome (5RS.5RL-4DL) and telosome 4DL formed metaphase I bonds at a frequency of 71%, demonstrating the significance of small terminal chromosome segments for pairing. This novel rye-wheat translocation is probably generated by homoeologous crossing-over because the distal region of 5RL is known to be homoeologous to that of 4DL. Possible association of this translocation with the absence of hairy peduncle character in Bronco 90 is discussed.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalEuphytica
Volume97
Issue number1
DOIs
Publication statusPublished - 1997

Fingerprint

triticale
hexaploidy
rye
Chromosomes
chromosomes
chromosome substitution
Metaphase
metaphase
Triticum
Chromosomes, Human, 13-15
Chromosome Pairing
wheat
crossing over
Bread
Constitution and Bylaws
chromosome banding
peduncle
sires
Triticale
Secale

Keywords

  • C-banding
  • Fluorescent in situ hybridization
  • Metaphase I bonds
  • Translocation
  • Triticale

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science

Cite this

Detection of a 4DL chromosome segment translocated to rye chromosome 5R in an advanced hexaploid triticale line Bronco 90. / Taketa, Shin; Nakazaki, Tetsuya; Schwarzacher, Trude; Heslop-Harrison, John S.

In: Euphytica, Vol. 97, No. 1, 1997, p. 91-96.

Research output: Contribution to journalArticle

Taketa, Shin ; Nakazaki, Tetsuya ; Schwarzacher, Trude ; Heslop-Harrison, John S. / Detection of a 4DL chromosome segment translocated to rye chromosome 5R in an advanced hexaploid triticale line Bronco 90. In: Euphytica. 1997 ; Vol. 97, No. 1. pp. 91-96.
@article{55632ddd40174e8b856f27e6f6dc3c0e,
title = "Detection of a 4DL chromosome segment translocated to rye chromosome 5R in an advanced hexaploid triticale line Bronco 90",
abstract = "Bronco 90 is an advanced line of hexaploid triticale and was reported to be a 2D(2R) chromosome substitution type. In F1 hybrids of this triticale with bread wheat, however, a meiotic configuration of 16 bivalents and 10 univalents was frequently observed indicating the presence of an additional D(R) chromosome substitution or D/R translocation. To determine the chromosome constitution of Bronco 90, C-banding and fluorescent in sire hybridization techniques were applied to somatic and meiotic metaphase chromosomes. These analyses revealed that in Bronco 90, the terminal 7{\%} of the long arm of rye chromosome 5R is derived from the long arm of chromosome 4D. This translocated chromosome (5RS.5RL-4DL) and telosome 4DL formed metaphase I bonds at a frequency of 71{\%}, demonstrating the significance of small terminal chromosome segments for pairing. This novel rye-wheat translocation is probably generated by homoeologous crossing-over because the distal region of 5RL is known to be homoeologous to that of 4DL. Possible association of this translocation with the absence of hairy peduncle character in Bronco 90 is discussed.",
keywords = "C-banding, Fluorescent in situ hybridization, Metaphase I bonds, Translocation, Triticale",
author = "Shin Taketa and Tetsuya Nakazaki and Trude Schwarzacher and Heslop-Harrison, {John S.}",
year = "1997",
doi = "10.1023/A:1003080720269",
language = "English",
volume = "97",
pages = "91--96",
journal = "Euphytica",
issn = "0014-2336",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Detection of a 4DL chromosome segment translocated to rye chromosome 5R in an advanced hexaploid triticale line Bronco 90

AU - Taketa, Shin

AU - Nakazaki, Tetsuya

AU - Schwarzacher, Trude

AU - Heslop-Harrison, John S.

PY - 1997

Y1 - 1997

N2 - Bronco 90 is an advanced line of hexaploid triticale and was reported to be a 2D(2R) chromosome substitution type. In F1 hybrids of this triticale with bread wheat, however, a meiotic configuration of 16 bivalents and 10 univalents was frequently observed indicating the presence of an additional D(R) chromosome substitution or D/R translocation. To determine the chromosome constitution of Bronco 90, C-banding and fluorescent in sire hybridization techniques were applied to somatic and meiotic metaphase chromosomes. These analyses revealed that in Bronco 90, the terminal 7% of the long arm of rye chromosome 5R is derived from the long arm of chromosome 4D. This translocated chromosome (5RS.5RL-4DL) and telosome 4DL formed metaphase I bonds at a frequency of 71%, demonstrating the significance of small terminal chromosome segments for pairing. This novel rye-wheat translocation is probably generated by homoeologous crossing-over because the distal region of 5RL is known to be homoeologous to that of 4DL. Possible association of this translocation with the absence of hairy peduncle character in Bronco 90 is discussed.

AB - Bronco 90 is an advanced line of hexaploid triticale and was reported to be a 2D(2R) chromosome substitution type. In F1 hybrids of this triticale with bread wheat, however, a meiotic configuration of 16 bivalents and 10 univalents was frequently observed indicating the presence of an additional D(R) chromosome substitution or D/R translocation. To determine the chromosome constitution of Bronco 90, C-banding and fluorescent in sire hybridization techniques were applied to somatic and meiotic metaphase chromosomes. These analyses revealed that in Bronco 90, the terminal 7% of the long arm of rye chromosome 5R is derived from the long arm of chromosome 4D. This translocated chromosome (5RS.5RL-4DL) and telosome 4DL formed metaphase I bonds at a frequency of 71%, demonstrating the significance of small terminal chromosome segments for pairing. This novel rye-wheat translocation is probably generated by homoeologous crossing-over because the distal region of 5RL is known to be homoeologous to that of 4DL. Possible association of this translocation with the absence of hairy peduncle character in Bronco 90 is discussed.

KW - C-banding

KW - Fluorescent in situ hybridization

KW - Metaphase I bonds

KW - Translocation

KW - Triticale

UR - http://www.scopus.com/inward/record.url?scp=0030856016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030856016&partnerID=8YFLogxK

U2 - 10.1023/A:1003080720269

DO - 10.1023/A:1003080720269

M3 - Article

VL - 97

SP - 91

EP - 96

JO - Euphytica

JF - Euphytica

SN - 0014-2336

IS - 1

ER -