Detection of 3-nitrotyrosine in atmospheric environments via a highperformance liquid chromatography-electrochemical detector system

Tatsuo Ito, Keiki Ogino, Kenjiro Nagaoka, Kei Takemoto, Rina Nishiyama, Yurika Shimizu

Research output: Contribution to journalArticle

Abstract

3-nitrotyrosine (3-NT) is generated from the tyrosine residue in atmospheric bio-aerosol proteins via a reaction with ozone (O 3 ) and nitrogen dioxide (NO 2 ). Stable 3-NT is a specific marker for oxidative damage and is reported to have a promotive effect to elicit allergies. In the present study, we report the development of a highly sensitive assay to quantify 3-NT in air sampler filters to collect < 2.5 µm of particulate matter (PM 2.5 ) from urban environmental air, including bio-aerosol. In this method, a 6 mm-diameter round hole was cut from the filters of air samplers and mixed with a nonspecific protease cocktail in order to hydrolyze proteins. Protein samples digested to the amino acid level were tested for 3-NT using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD). The maximum 3-NT content was detected in a prefilter for PM of sizes from 4.5 to 7.3 µm, with a detection limit of 1.13 pg/m 3 .

Original languageEnglish
Article numbere58371
JournalJournal of Visualized Experiments
Volume2019
Issue number143
DOIs
Publication statusPublished - Jan 2019

Keywords

  • 3-nitrotyrosine
  • Air atmospheric environmental pollution
  • Environmental sciences
  • HPLC-ECD
  • Issue 143
  • Nitrogen dioxide
  • Ozone
  • Particulate matter
  • Protein modification

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Detection of 3-nitrotyrosine in atmospheric environments via a highperformance liquid chromatography-electrochemical detector system'. Together they form a unique fingerprint.

  • Cite this