Abstract
A concentration detection limit of 100 fM was achieved for the fluorescein ion by improving the experimental setup used for Hadamard transform capillary electrophoresis. Two argon-ion lasers, a gating laser for sample injection and a probe laser for the excitation of analyte molecules, were employed for the efficient photodegradation of analyte molecules in laser-induced fluorescence detection using an optically gated sample-injection method. In addition, a dichroic mirror, located in the pathway of the probe laser was used to exclude the other lines of the argon-ion laser. Using a Hadamard matrix on the order of 2046, the concentration limit of detection for fluorescein ion was determined to be 100 fM at S/N = 3, in which the average number of molecules in a single injection volume was calculated to be 27. The influences of the output power in both the gating and probe lasers on the sensitivity are also discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 1659-1661 |
| Number of pages | 3 |
| Journal | Analytical Sciences |
| Volume | 19 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2003 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Detection of 27 Molecules in a Single Injection Volume by Hadamard Transform Capillary Electrophoresis. / Kaneta, Takashi; Kosai, Kazuki; Imasaka, Totaro.
In: Analytical Sciences, Vol. 19, No. 12, 12.2003, p. 1659-1661.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of 27 Molecules in a Single Injection Volume by Hadamard Transform Capillary Electrophoresis
AU - Kaneta, Takashi
AU - Kosai, Kazuki
AU - Imasaka, Totaro
PY - 2003/12
Y1 - 2003/12
N2 - A concentration detection limit of 100 fM was achieved for the fluorescein ion by improving the experimental setup used for Hadamard transform capillary electrophoresis. Two argon-ion lasers, a gating laser for sample injection and a probe laser for the excitation of analyte molecules, were employed for the efficient photodegradation of analyte molecules in laser-induced fluorescence detection using an optically gated sample-injection method. In addition, a dichroic mirror, located in the pathway of the probe laser was used to exclude the other lines of the argon-ion laser. Using a Hadamard matrix on the order of 2046, the concentration limit of detection for fluorescein ion was determined to be 100 fM at S/N = 3, in which the average number of molecules in a single injection volume was calculated to be 27. The influences of the output power in both the gating and probe lasers on the sensitivity are also discussed.
AB - A concentration detection limit of 100 fM was achieved for the fluorescein ion by improving the experimental setup used for Hadamard transform capillary electrophoresis. Two argon-ion lasers, a gating laser for sample injection and a probe laser for the excitation of analyte molecules, were employed for the efficient photodegradation of analyte molecules in laser-induced fluorescence detection using an optically gated sample-injection method. In addition, a dichroic mirror, located in the pathway of the probe laser was used to exclude the other lines of the argon-ion laser. Using a Hadamard matrix on the order of 2046, the concentration limit of detection for fluorescein ion was determined to be 100 fM at S/N = 3, in which the average number of molecules in a single injection volume was calculated to be 27. The influences of the output power in both the gating and probe lasers on the sensitivity are also discussed.
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U2 - 10.2116/analsci.19.1659
DO - 10.2116/analsci.19.1659
M3 - Article
VL - 19
SP - 1659
EP - 1661
JO - Analytical Sciences
JF - Analytical Sciences
SN - 0910-6340
IS - 12
ER -