Detection by western blotting of an antibody to the hepatitis C virus E1 envelope protein in sera of patients with chronic liver disease.

H. Hada, N. Koide, T. Hanafusa, K. Sakaguchi, T. Shinji, S. Sasaki, T. Oka, N. Takayama, Y. Yumoto, T. Tsuji

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We detected an antibody to HCV envelope protein (E1) in sera of patients with HCV-related chronic liver diseases (20 patients with chronic hepatitis and 5 patients with liver cirrhosis) by Western blotting using the fusion protein of E1 envelope protein and beta-galactosidase as an antigen. The antibody to HCV E1 (anti-HCV E1) was detected in 8 (42%) of 19 patients positive for HCV-RNA (16 were positive and 3 were negative for antibody to C100-3) and in 1 (17%) of 6 patients negative for HCV-RNA but positive for antibody to C100-3. HCV-RNA was detected in 8 (89%) of 9 anti-HCV E1 positive sera. The value of alanine aminotransferase was significantly higher in patients positive for anti-HCV E1 than in patients negative for the antibody. Although an antibody to the envelope protein of HCV is suspected to be one of the candidates of virus-neutralizing antibodies, our results suggest this hypothesis appears to be unlikely.

Original languageEnglish
Pages (from-to)365-370
Number of pages6
JournalActa medica Okayama
Volume46
Issue number5
Publication statusPublished - Oct 1992

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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