Detection and serotype distribution of Actinobacillus actinomycetemcomitans in cardiovascular specimens from Japanese patients

K. Nakano, Hiroaki Inaba, R. Nomura, H. Nemoto, K. Tamura, E. Miyamoto, H. Yoshioka, K. Taniguchi, A. Amano, T. Ooshima

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Actinobacillus actinomycetemcomitans, an important pathogen in periodontitis, has also been detected in cardiovascular tissues. Sixty heart valves were collected during valve replacement surgery from 60 patients (one from each), 10 were from patients with infective endocarditis (IE group) and 50 were from patients with other valvular diseases (non-IE group). In addition, 46 samples of aneurysmal tissue were taken from 46 patients with a thoracic or abdominal aneurysm (Aneurysm group, one from each). Dental plaque samples were taken from 54 of the patients, 31 in the IE and non-IE groups and 23 in the aneurysm group. First, the distribution of A. actinomycetemcomitans in all specimens was analysed using a polymerase chain reaction method, which resulted in a positive reaction in 33 (31.1%) of the cardiovascular specimens and 25 (46.3%) of the dental plaque samples. Next, using serotype-specific sets of primers, the serotype distribution of A. actinomycetemcomitans in the cardiovascular specimens and dental plaque samples was found to be significantly different compared to dental plaque samples from Japanese subjects reported previously.

Original languageEnglish
Pages (from-to)136-139
Number of pages4
JournalOral Microbiology and Immunology
Volume22
Issue number2
DOIs
Publication statusPublished - Apr 2007
Externally publishedYes

Fingerprint

Aggregatibacter actinomycetemcomitans
Dental Plaque
Aneurysm
Periodontitis
Heart Valves
Endocarditis
Thorax
Serogroup
Polymerase Chain Reaction

Keywords

  • Actinobacillus actinomycetemcomitans
  • Cardiovascular specimens
  • Polymerase chain reaction
  • Serotype

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Detection and serotype distribution of Actinobacillus actinomycetemcomitans in cardiovascular specimens from Japanese patients. / Nakano, K.; Inaba, Hiroaki; Nomura, R.; Nemoto, H.; Tamura, K.; Miyamoto, E.; Yoshioka, H.; Taniguchi, K.; Amano, A.; Ooshima, T.

In: Oral Microbiology and Immunology, Vol. 22, No. 2, 04.2007, p. 136-139.

Research output: Contribution to journalArticle

Nakano, K, Inaba, H, Nomura, R, Nemoto, H, Tamura, K, Miyamoto, E, Yoshioka, H, Taniguchi, K, Amano, A & Ooshima, T 2007, 'Detection and serotype distribution of Actinobacillus actinomycetemcomitans in cardiovascular specimens from Japanese patients', Oral Microbiology and Immunology, vol. 22, no. 2, pp. 136-139. https://doi.org/10.1111/j.1399-302X.2007.00332.x
Nakano, K. ; Inaba, Hiroaki ; Nomura, R. ; Nemoto, H. ; Tamura, K. ; Miyamoto, E. ; Yoshioka, H. ; Taniguchi, K. ; Amano, A. ; Ooshima, T. / Detection and serotype distribution of Actinobacillus actinomycetemcomitans in cardiovascular specimens from Japanese patients. In: Oral Microbiology and Immunology. 2007 ; Vol. 22, No. 2. pp. 136-139.
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