Abstract
Although δ-crystallin (d-crys), also known as lens protein, is transiently expressed in Rathke's pouch (RP) of the chick embryo, detailed temporal and spatial expression patterns have been obscure. In this study, to understand the relationship between the δ-crys mRNAexpressing region and RP formation, we examined the embryonic expression pattern of δ-crys mRNA in the primordium of the adenohypophysis. δ-crys mRNA expression was initially found at stage 15 anterior to the foregut and posterior to the invaginated oral ectoderm. After RP formation, the δ-crys mRNA was expressed in the postventral region of RP and the anterior region of RP. δ-crys mRNA expression was then restricted to the cephalic lobe of the pituitary gland. From stage 20, the δ-crys and alphaglycoprotein subunit (αGSU) mRNA-expressing regions were almost completely overlapping. The αGSU mRNAexpressing region is thought to be the primordium of the pars tuberalis, and these regions were overlapped with the Lhx3 mRNA-expressing region. The intensity of δ-crys mRNA expression gradually decreased with development and completely disappeared by stage 34. These results suggest that the embryonic chick pituitary gland consists of two different regions labeled with δ-crys and Lhx3.
| Original language | English |
|---|---|
| Pages (from-to) | 273-280 |
| Number of pages | 8 |
| Journal | Journal of Molecular Histology |
| Volume | 43 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 2012 |
| Externally published | Yes |
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Keywords
- αGSU
- δ-crystallin
- Chicken
- Pituitary gland
- Tuberalis
ASJC Scopus subject areas
- Cell Biology
- Histology
- Physiology
Cite this
Detailed analysis of the δ-crystallin mRNA-expressing region in early development of the chick pituitary gland. / Inoue, Makiko; Shiina, Tomoya; Aizawa, Sayaka; Sakata, Ichiro; Takagi, Hiroyasu; Sakai, Takafumi.
In: Journal of Molecular Histology, Vol. 43, No. 3, 06.2012, p. 273-280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detailed analysis of the δ-crystallin mRNA-expressing region in early development of the chick pituitary gland
AU - Inoue, Makiko
AU - Shiina, Tomoya
AU - Aizawa, Sayaka
AU - Sakata, Ichiro
AU - Takagi, Hiroyasu
AU - Sakai, Takafumi
PY - 2012/6
Y1 - 2012/6
N2 - Although δ-crystallin (d-crys), also known as lens protein, is transiently expressed in Rathke's pouch (RP) of the chick embryo, detailed temporal and spatial expression patterns have been obscure. In this study, to understand the relationship between the δ-crys mRNAexpressing region and RP formation, we examined the embryonic expression pattern of δ-crys mRNA in the primordium of the adenohypophysis. δ-crys mRNA expression was initially found at stage 15 anterior to the foregut and posterior to the invaginated oral ectoderm. After RP formation, the δ-crys mRNA was expressed in the postventral region of RP and the anterior region of RP. δ-crys mRNA expression was then restricted to the cephalic lobe of the pituitary gland. From stage 20, the δ-crys and alphaglycoprotein subunit (αGSU) mRNA-expressing regions were almost completely overlapping. The αGSU mRNAexpressing region is thought to be the primordium of the pars tuberalis, and these regions were overlapped with the Lhx3 mRNA-expressing region. The intensity of δ-crys mRNA expression gradually decreased with development and completely disappeared by stage 34. These results suggest that the embryonic chick pituitary gland consists of two different regions labeled with δ-crys and Lhx3.
AB - Although δ-crystallin (d-crys), also known as lens protein, is transiently expressed in Rathke's pouch (RP) of the chick embryo, detailed temporal and spatial expression patterns have been obscure. In this study, to understand the relationship between the δ-crys mRNAexpressing region and RP formation, we examined the embryonic expression pattern of δ-crys mRNA in the primordium of the adenohypophysis. δ-crys mRNA expression was initially found at stage 15 anterior to the foregut and posterior to the invaginated oral ectoderm. After RP formation, the δ-crys mRNA was expressed in the postventral region of RP and the anterior region of RP. δ-crys mRNA expression was then restricted to the cephalic lobe of the pituitary gland. From stage 20, the δ-crys and alphaglycoprotein subunit (αGSU) mRNA-expressing regions were almost completely overlapping. The αGSU mRNAexpressing region is thought to be the primordium of the pars tuberalis, and these regions were overlapped with the Lhx3 mRNA-expressing region. The intensity of δ-crys mRNA expression gradually decreased with development and completely disappeared by stage 34. These results suggest that the embryonic chick pituitary gland consists of two different regions labeled with δ-crys and Lhx3.
KW - αGSU
KW - δ-crystallin
KW - Chicken
KW - Pituitary gland
KW - Tuberalis
UR - http://www.scopus.com/inward/record.url?scp=84865629395&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865629395&partnerID=8YFLogxK
U2 - 10.1007/s10735-012-9407-1
DO - 10.1007/s10735-012-9407-1
M3 - Article
C2 - 22461196
AN - SCOPUS:84865629395
VL - 43
SP - 273
EP - 280
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
SN - 1567-2379
IS - 3
ER -