Detailed analysis of the δ-crystallin mRNA-expressing region in early development of the chick pituitary gland

Makiko Inoue, Tomoya Shiina, Sayaka Aizawa, Ichiro Sakata, Hiroyasu Takagi, Takafumi Sakai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Although δ-crystallin (d-crys), also known as lens protein, is transiently expressed in Rathke's pouch (RP) of the chick embryo, detailed temporal and spatial expression patterns have been obscure. In this study, to understand the relationship between the δ-crys mRNAexpressing region and RP formation, we examined the embryonic expression pattern of δ-crys mRNA in the primordium of the adenohypophysis. δ-crys mRNA expression was initially found at stage 15 anterior to the foregut and posterior to the invaginated oral ectoderm. After RP formation, the δ-crys mRNA was expressed in the postventral region of RP and the anterior region of RP. δ-crys mRNA expression was then restricted to the cephalic lobe of the pituitary gland. From stage 20, the δ-crys and alphaglycoprotein subunit (αGSU) mRNA-expressing regions were almost completely overlapping. The αGSU mRNAexpressing region is thought to be the primordium of the pars tuberalis, and these regions were overlapped with the Lhx3 mRNA-expressing region. The intensity of δ-crys mRNA expression gradually decreased with development and completely disappeared by stage 34. These results suggest that the embryonic chick pituitary gland consists of two different regions labeled with δ-crys and Lhx3.

Original languageEnglish
Pages (from-to)273-280
Number of pages8
JournalJournal of Molecular Histology
Volume43
Issue number3
DOIs
Publication statusPublished - Jun 2012
Externally publishedYes

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Crystallins
Pituitary Gland
Messenger RNA
Ectoderm
Anterior Pituitary Gland
Chick Embryo
Head

Keywords

  • αGSU
  • δ-crystallin
  • Chicken
  • Pituitary gland
  • Tuberalis

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Physiology

Cite this

Detailed analysis of the δ-crystallin mRNA-expressing region in early development of the chick pituitary gland. / Inoue, Makiko; Shiina, Tomoya; Aizawa, Sayaka; Sakata, Ichiro; Takagi, Hiroyasu; Sakai, Takafumi.

In: Journal of Molecular Histology, Vol. 43, No. 3, 06.2012, p. 273-280.

Research output: Contribution to journalArticle

Inoue, Makiko ; Shiina, Tomoya ; Aizawa, Sayaka ; Sakata, Ichiro ; Takagi, Hiroyasu ; Sakai, Takafumi. / Detailed analysis of the δ-crystallin mRNA-expressing region in early development of the chick pituitary gland. In: Journal of Molecular Histology. 2012 ; Vol. 43, No. 3. pp. 273-280.
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