Detachment of chain-forming neuroblasts by Fyn-mediated control of cell– cell adhesion in the postnatal brain

Kazuma Fujikake, Masato Sawada, Takao Hikita, Yayoi Seto, Naoko Kaneko, Vicente Herranz-Pérez, Natsuki Dohi, Natsumi Homma, Satoshi Osaga, Yuchio Yanagawa, Toshihiro Akaike, Jose Manuel García-Verdugo, Mitsuharu Hattori, Kazuya Sobue, Kazunobu Sawamoto

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

In the rodent olfactory system, neuroblasts produced in the ventricular-subventricular zone of the postnatal brain migrate tangentially in chain-like cell aggregates toward the olfactory bulb (OB) through the rostral migratory stream (RMS). After reaching the OB, the chains are dissociated and the neuroblasts migrate individually and radially toward their final destination. The cellular and molecular mechanisms controlling cell– cell adhesion during this detachment remain unclear. Here we report that Fyn, a nonreceptor tyrosine kinase, regulates the detachment of neuroblasts from chains in the male and female mouse OB. By performing chemical screening and in vivo loss-of-function and gain-of-function experiments, we found that Fyn promotes somal disengagement from the chains and is involved in neuronal migration from the RMS into the granule cell layer of the OB. Fyn knockdown or Dab1 (disabled-1) deficiency caused p120- catenin to accumulate and adherens junction-like structures to be sustained at the contact sites between neuroblasts. Moreover, a Fyn and N-cadherin double-knockdown experiment indicated that Fyn regulates the N-cadherin-mediated cell adhesion between neuroblasts. These results suggest that the Fyn-mediated control of cell– cell adhesion is critical for the detachment of chain-forming neuroblasts in the postnatal OB.

Original languageEnglish
Pages (from-to)4598-4609
Number of pages12
JournalJournal of Neuroscience
Volume38
Issue number19
DOIs
Publication statusPublished - May 9 2018
Externally publishedYes

Keywords

  • Adhesion
  • Neuronal migration
  • Olfactory bulb
  • Rostral migratory stream

ASJC Scopus subject areas

  • Medicine(all)

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