Desmosomal proteins in cultured and intact human periodontal ligament fibroblasts

Y. Yamaoka, Y. Sawa, N. Ebata, S. Yoshida, T. Kawasaki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This study examined the kinds of desmosomal proteins in the human periodontal ligament fibroblasts (PDLFs). The PDLFs obtained from young and older patients were cultured and the amounts of desmosomal proteins were measured by ELISA with antibodies to desmoplakins, desmogleins, and desmocollins. Cultured cells and tissue sections of the human periodontal ligament were immunostained with the same antibodies. Expression of desmosomal proteins in the PDLFs was clearly demonstrated both by ELISA and the immunohistochemical studies, suggesting the existence of desmosome-like junctions in the PDLFs. The junctions are considered to protect gap junctions in the PDLFs against cell transformation caused by cell contraction, which may relate to tooth eruption and repair of periodontal tissue, and/or strong occlusal forces. Statistically significant differences (P<0.0001) in the expression of desmoplakins and desmogleins between younger and older patients were observed in this study.

Original languageEnglish
Pages (from-to)605-609
Number of pages5
JournalTissue and Cell
Volume31
Issue number6
DOIs
Publication statusPublished - Dec 1999
Externally publishedYes

Keywords

  • Desmocollin
  • Desmoglein
  • Desmoplakin
  • Desmosome
  • Fibroblast
  • Periodontal ligament

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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