Desmoplastic Reaction in 3D-Pancreatic Cancer Tissues Suppresses Molecular Permeability

Michiya Matsusaki, Misaki Komeda, Simona Mura, Hiroyoshi Tanaka, Mitsunobu Kano, Patrick Couvreur, Mitsuru Akashi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The survival rate of pancreatic ductal adenocarcinoma is still the lowest among all types of cancers, primarily as a consequence of an important desmoplastic reaction. Although the presence of thick stromal tissues in pancreatic tumors has been reported, in vivo animal studies do not enable a clear understanding of the crosstalk between cancer cells and fibroblasts. Accordingly, this paper reports the design and characterization of an in vitro pancreatic cancer-stromal 3D-tissue model, which enhances the understanding of the interactions between cancer cells and fibroblasts and their influence on the secretion of extracellular matrix (ECM). 3D-tissue models comprising fibroblasts and pancreatic cancer cells (MiaPaCa-2 cell line) or colon cancer cells (HT29 cell line, used as a control) show decreased molecular permeability with increased cancer cell ratios. The 3D-MiaPaCa-2 tissues display an increase in the secretion of collagen as a function of the cancer cell ratio, whereas 3D-HT29 tissues do not show a significant difference. Notably, the secretion of ECM proteins from single fibroblasts in 3D-tissue models containing 90% MiaPaCa-2 cells is ten times higher than that under 10% cancer cell conditions. In vitro pancreatic cancer 3D-tissues will be a valuable tool to obtain information on the interactions between cancer and stromal cells.

Original languageEnglish
JournalAdvanced healthcare materials
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Pancreatic Neoplasms
Permeability
Cells
Tissue
Fibroblasts
Neoplasms
Cell Line
HT29 Cells
Extracellular Matrix Proteins
Stromal Cells
Colonic Neoplasms
Crosstalk
Extracellular Matrix
Collagen
Adenocarcinoma
Tumors
Animals
Proteins

Keywords

  • Extracellular matrix secretion
  • Pancreatic cancer
  • Permeability
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

Desmoplastic Reaction in 3D-Pancreatic Cancer Tissues Suppresses Molecular Permeability. / Matsusaki, Michiya; Komeda, Misaki; Mura, Simona; Tanaka, Hiroyoshi; Kano, Mitsunobu; Couvreur, Patrick; Akashi, Mitsuru.

In: Advanced healthcare materials, 2017.

Research output: Contribution to journalArticle

Matsusaki, Michiya ; Komeda, Misaki ; Mura, Simona ; Tanaka, Hiroyoshi ; Kano, Mitsunobu ; Couvreur, Patrick ; Akashi, Mitsuru. / Desmoplastic Reaction in 3D-Pancreatic Cancer Tissues Suppresses Molecular Permeability. In: Advanced healthcare materials. 2017.
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