Desmoplakin as a specific marker of lymphatic vessels

Noriyuki Ebata, Yoshinobu Nodasaka, Yoshihiko Sawa, Yuji Yamaoka, Shujiro Makino, Yasunori Totsuka, Shigemitsu Yoshida

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The usefulness of immunostaining with anti-desmoplakin antibody for light microscopic identification of lymphatic vessels was examined in cryostat sections of the human tongue. The results were compared with laminin, 5′-nucleotidase (5′-Nase), and factor VIII staining. Immunoelectron microscopic observation was also performed to confirm that the vessels reacting with anti-desmoplakin were lymphatic vessels. Under the immunoelectron microscopic, the vessels reacting with anti-desmoplakin showed ultrastructural features characteristic of lymphatic vessels: thin endothelial walls, no or incomplete basal lamina, open junctions, and overlapping endothelium. In general, lymphatic vessels identified by anti-desmoplakin reacted strongly with 5′-Nase, but showed weak or no reactivity with anti-laminin and anti-factor VIII. Blood vessels showed no reactivity with anti-desmoplakin, but reacted strongly with anti-laminin and anti-factor VIII. However, some blood and lymphatic vessels showed intermediate reactivity with anti-laminin, anti-factor VIII, and 5′-Nase. It was difficult to identify these as blood or lymphatic vessels only by the reactivity differences. The results indicate that anti-desmoplakin antibody specifically distinguishes lymphatic vessels and is useful for studying the fine distribution of lymphatic vessels under light microscopy.

Original languageEnglish
Pages (from-to)40-48
Number of pages9
JournalMicrovascular Research
Volume61
Issue number1
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

Keywords

  • 5′-Nase
  • Desmoplakin
  • Factor VIII
  • Laminin
  • Lymphatic vessel

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

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  • Cite this

    Ebata, N., Nodasaka, Y., Sawa, Y., Yamaoka, Y., Makino, S., Totsuka, Y., & Yoshida, S. (2001). Desmoplakin as a specific marker of lymphatic vessels. Microvascular Research, 61(1), 40-48. https://doi.org/10.1006/mvre.2000.2280