Desmoplakin as a specific marker of lymphatic vessels

Noriyuki Ebata, Yoshinobu Nodasaka, Yoshihiko Sawa, Yuji Yamaoka, Shujiro Makino, Yasunori Totsuka, Shigemitsu Yoshida

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The usefulness of immunostaining with anti-desmoplakin antibody for light microscopic identification of lymphatic vessels was examined in cryostat sections of the human tongue. The results were compared with laminin, 5′-nucleotidase (5′-Nase), and factor VIII staining. Immunoelectron microscopic observation was also performed to confirm that the vessels reacting with anti-desmoplakin were lymphatic vessels. Under the immunoelectron microscopic, the vessels reacting with anti-desmoplakin showed ultrastructural features characteristic of lymphatic vessels: thin endothelial walls, no or incomplete basal lamina, open junctions, and overlapping endothelium. In general, lymphatic vessels identified by anti-desmoplakin reacted strongly with 5′-Nase, but showed weak or no reactivity with anti-laminin and anti-factor VIII. Blood vessels showed no reactivity with anti-desmoplakin, but reacted strongly with anti-laminin and anti-factor VIII. However, some blood and lymphatic vessels showed intermediate reactivity with anti-laminin, anti-factor VIII, and 5′-Nase. It was difficult to identify these as blood or lymphatic vessels only by the reactivity differences. The results indicate that anti-desmoplakin antibody specifically distinguishes lymphatic vessels and is useful for studying the fine distribution of lymphatic vessels under light microscopy.

Original languageEnglish
Pages (from-to)40-48
Number of pages9
JournalMicrovascular Research
Volume61
Issue number1
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Desmoplakins
Lymphatic Vessels
Factor VIII
5'-Nucleotidase
Laminin
Blood Vessels
Blood
Anti-Idiotypic Antibodies
Cryostats
Antibodies
Blood vessels
Light
Optical microscopy
Identification (control systems)
Tongue
Basement Membrane
Endothelium
Microscopy
Staining and Labeling

Keywords

  • 5′-Nase
  • Desmoplakin
  • Factor VIII
  • Laminin
  • Lymphatic vessel

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Ebata, N., Nodasaka, Y., Sawa, Y., Yamaoka, Y., Makino, S., Totsuka, Y., & Yoshida, S. (2001). Desmoplakin as a specific marker of lymphatic vessels. Microvascular Research, 61(1), 40-48. https://doi.org/10.1006/mvre.2000.2280

Desmoplakin as a specific marker of lymphatic vessels. / Ebata, Noriyuki; Nodasaka, Yoshinobu; Sawa, Yoshihiko; Yamaoka, Yuji; Makino, Shujiro; Totsuka, Yasunori; Yoshida, Shigemitsu.

In: Microvascular Research, Vol. 61, No. 1, 2001, p. 40-48.

Research output: Contribution to journalArticle

Ebata, N, Nodasaka, Y, Sawa, Y, Yamaoka, Y, Makino, S, Totsuka, Y & Yoshida, S 2001, 'Desmoplakin as a specific marker of lymphatic vessels', Microvascular Research, vol. 61, no. 1, pp. 40-48. https://doi.org/10.1006/mvre.2000.2280
Ebata N, Nodasaka Y, Sawa Y, Yamaoka Y, Makino S, Totsuka Y et al. Desmoplakin as a specific marker of lymphatic vessels. Microvascular Research. 2001;61(1):40-48. https://doi.org/10.1006/mvre.2000.2280
Ebata, Noriyuki ; Nodasaka, Yoshinobu ; Sawa, Yoshihiko ; Yamaoka, Yuji ; Makino, Shujiro ; Totsuka, Yasunori ; Yoshida, Shigemitsu. / Desmoplakin as a specific marker of lymphatic vessels. In: Microvascular Research. 2001 ; Vol. 61, No. 1. pp. 40-48.
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