Design, synthesis, and preliminary ex vivo and in vivo evaluation of cationic magnetic resonance contrast agent for rabbit articular cartilage imaging

Takayoshi Irie, Kazuhiro Oda, Akihiko Shiino, Mitsuhiko Kubo, Shigehiro Morikawa, Noboru Urushiyama, Shuji Aonuma, Takahide Kimura, Toshiro Inubushi, Toshitaka Oohashi, Naoki Komatsu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Since early-stage diagnosis of osteoarthritis (OA) is necessary to retard the progress of the disease, magnetic resonance (MR) and computed tomography (CT) imaging agents have been applied to evaluate the integrity of the extracellular matrix (ECM) in the articular cartilage (AC). Although several negatively charged contrast agents were employed, they provided indirect images of the AC based on the coulombic repulsion with anionic glycosaminoglycans (GAGs) in the ECM. To achieve direct imaging of the ECM, positively charged contrast agents have quite recently been designed for optical and CT imaging. In this paper, we report on a positively charged MR contrast agent, DOTA-Gd-G 2R8, which was applied preliminarily to ex vivo and in vivo imaging of rabbit AC. In the ex vivo imaging, the contrast agent was accumulated in the AC in high concentration due to the strong coulombic attraction between the negative charge of the GAGs and the positive charge of the octaarginine (R8). The thickness of the AC measured in the MR image was found to be comparable to that determined by the histological staining of the slice. The AC was also observed in vivo by MR imaging with the use of DOTA-Gd-G2R8.

Original languageEnglish
Pages (from-to)1508-1512
Number of pages5
JournalMedChemComm
Volume4
Issue number11
DOIs
Publication statusPublished - Nov 2013

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Cartilage
Articular Cartilage
Magnetic resonance
Contrast Media
Magnetic Resonance Spectroscopy
Rabbits
Imaging techniques
Extracellular Matrix
Glycosaminoglycans
Tomography
Optical Tomography
Osteoarthritis
Early Diagnosis
Magnetic Resonance Imaging
Staining and Labeling

ASJC Scopus subject areas

  • Biochemistry
  • Pharmaceutical Science

Cite this

Design, synthesis, and preliminary ex vivo and in vivo evaluation of cationic magnetic resonance contrast agent for rabbit articular cartilage imaging. / Irie, Takayoshi; Oda, Kazuhiro; Shiino, Akihiko; Kubo, Mitsuhiko; Morikawa, Shigehiro; Urushiyama, Noboru; Aonuma, Shuji; Kimura, Takahide; Inubushi, Toshiro; Oohashi, Toshitaka; Komatsu, Naoki.

In: MedChemComm, Vol. 4, No. 11, 11.2013, p. 1508-1512.

Research output: Contribution to journalArticle

Irie, T, Oda, K, Shiino, A, Kubo, M, Morikawa, S, Urushiyama, N, Aonuma, S, Kimura, T, Inubushi, T, Oohashi, T & Komatsu, N 2013, 'Design, synthesis, and preliminary ex vivo and in vivo evaluation of cationic magnetic resonance contrast agent for rabbit articular cartilage imaging', MedChemComm, vol. 4, no. 11, pp. 1508-1512. https://doi.org/10.1039/c3md00229b
Irie, Takayoshi ; Oda, Kazuhiro ; Shiino, Akihiko ; Kubo, Mitsuhiko ; Morikawa, Shigehiro ; Urushiyama, Noboru ; Aonuma, Shuji ; Kimura, Takahide ; Inubushi, Toshiro ; Oohashi, Toshitaka ; Komatsu, Naoki. / Design, synthesis, and preliminary ex vivo and in vivo evaluation of cationic magnetic resonance contrast agent for rabbit articular cartilage imaging. In: MedChemComm. 2013 ; Vol. 4, No. 11. pp. 1508-1512.
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