Design of Pyrene-Fatty Acid Conjugates for Real-Time Monitoring of Drug Delivery and Controllability of Drug Release

Keita Hayashi, Yuma Mitsuyoshi, Toshiyuki Kamei, Toshinori Shimanouchi, Keishi Suga, Yukihiro Okamoto, Hidemi Nakamura, Hiroshi Umakoshi

Research output: Contribution to journalArticle

Abstract

Fluorescence probes are usually employed to analyze pharmacokinetics of drug carriers; however, this method using usual probes is not suitable to monitor drug carriers in detail because fluorescence spectra do not change by the disruption of drug carriers. In this study, pyrene-fatty acid conjugates were investigated as probes to monitor the state of drug carriers in real time. 1-Pyrenemethanol was conjugated with fatty acids, such as lauric acid, stearic acid, and behenic acid, and the conjugates were stirred in ethanol, resulting in the formation of submicron particles; these particles exhibited excimer emission. When J774.1 and Colon 26 cells were treated with these particles, the associated fluorescence spectra shifted from excimer emission to monomer emission. Moreover, the degree of change was controlled by the type of fatty acid. These results support the design of drug carriers that can be used to monitor pharmacokinetics in real time and to control the disruption time.

Original languageEnglish
Pages (from-to)3572-3580
Number of pages9
JournalACS Omega
Volume3
Issue number3
DOIs
Publication statusPublished - Jan 1 2018

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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