Abstract
Thermolysin-catalyzed condensation of N - (benzylxycarbyl) - glycyl - glycyl - L -phenylalanyl-L-leucine ethyl ester (Z-Gly-GlyPheLeuOEt), the precursor of the des-TyrMeucine enkephalin from N-(benzyl-oxycarbonyl)-glycyl-glycine (Z-GlyGly) and L-phenylalanyl-L-leucine ethyl ester (PheLeuOEt) was elucidated as a model reaction for the enzymatic synthesis of oligopeptides with many side reactions. In buffer solution, the yield of Z-GlyGly-PheLeuOEt was extremely low with considerable by-products. A large increase in both yield and purity became possible in the aqueous-organic biphasic system by the systematic selection of experimental conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 452-454 |
| Number of pages | 3 |
| Journal | Bio/Technology |
| Volume | 4 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
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Design of proteinase-cataiyzed synthesis of oligopeptides in an aqueous-organic biphasic system. / Nakanishi, Kazuhiro; Kimura, Yukitaka; Matsuno, Ryuichi.
In: Bio/Technology, Vol. 4, No. 5, 1986, p. 452-454.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Design of proteinase-cataiyzed synthesis of oligopeptides in an aqueous-organic biphasic system
AU - Nakanishi, Kazuhiro
AU - Kimura, Yukitaka
AU - Matsuno, Ryuichi
PY - 1986
Y1 - 1986
N2 - Thermolysin-catalyzed condensation of N - (benzylxycarbyl) - glycyl - glycyl - L -phenylalanyl-L-leucine ethyl ester (Z-Gly-GlyPheLeuOEt), the precursor of the des-TyrMeucine enkephalin from N-(benzyl-oxycarbonyl)-glycyl-glycine (Z-GlyGly) and L-phenylalanyl-L-leucine ethyl ester (PheLeuOEt) was elucidated as a model reaction for the enzymatic synthesis of oligopeptides with many side reactions. In buffer solution, the yield of Z-GlyGly-PheLeuOEt was extremely low with considerable by-products. A large increase in both yield and purity became possible in the aqueous-organic biphasic system by the systematic selection of experimental conditions.
AB - Thermolysin-catalyzed condensation of N - (benzylxycarbyl) - glycyl - glycyl - L -phenylalanyl-L-leucine ethyl ester (Z-Gly-GlyPheLeuOEt), the precursor of the des-TyrMeucine enkephalin from N-(benzyl-oxycarbonyl)-glycyl-glycine (Z-GlyGly) and L-phenylalanyl-L-leucine ethyl ester (PheLeuOEt) was elucidated as a model reaction for the enzymatic synthesis of oligopeptides with many side reactions. In buffer solution, the yield of Z-GlyGly-PheLeuOEt was extremely low with considerable by-products. A large increase in both yield and purity became possible in the aqueous-organic biphasic system by the systematic selection of experimental conditions.
UR - http://www.scopus.com/inward/record.url?scp=84966196737&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84966196737&partnerID=8YFLogxK
U2 - 10.1038/nbt0586-452
DO - 10.1038/nbt0586-452
M3 - Article
VL - 4
SP - 452
EP - 454
JO - Biotechnology
JF - Biotechnology
SN - 0733-222X
IS - 5
ER -