Abstract
Thermolysin-catalyzed condensation of N - (benzylxycarbyl) - glycyl - glycyl - L -phenylalanyl-L-leucine ethyl ester (Z-Gly-GlyPheLeuOEt), the precursor of the des-TyrMeucine enkephalin from N-(benzyl-oxycarbonyl)-glycyl-glycine (Z-GlyGly) and L-phenylalanyl-L-leucine ethyl ester (PheLeuOEt) was elucidated as a model reaction for the enzymatic synthesis of oligopeptides with many side reactions. In buffer solution, the yield of Z-GlyGly-PheLeuOEt was extremely low with considerable by-products. A large increase in both yield and purity became possible in the aqueous-organic biphasic system by the systematic selection of experimental conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 452-454 |
| Number of pages | 3 |
| Journal | Bio/Technology |
| Volume | 4 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1986 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biotechnology
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Nakanishi, K., Kimura, Y., & Matsuno, R. (1986). Design of proteinase-cataiyzed synthesis of oligopeptides in an aqueous-organic biphasic system. Bio/Technology, 4(5), 452-454. https://doi.org/10.1038/nbt0586-452