Des-γ-carboxyl prothrombin is associated with tumor angiogenesis in hepatocellular carcinoma

Minoru Matsubara, Hidenori Shiraha, Jyunro Kataoka, Masaya Iwamuro, Shigeru Horiguchi, Shin Ichi Nishina, Nobuyuki Takaoka, Masayuki Uemura, Akinobu Takaki, Shinichiro Nakamura, Yoshiyuki Kobayashi, Kazuhiro Nouso, Kazuhide Yamamoto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background and Aim: Hepatocellular carcinoma (HCC) is a hypervascular tumor, and angiogenesis plays an important role in its development. Previously, we demonstrated that des-γ-carboxyl prothrombin (DCP) promotes both cell proliferation and migration of human umbilical vein endothelial cells (HUVECs) by inducing the autophosphorylation of kinase insert domain receptor (KDR). In the present study, DCP-associated tumor angiogenesis was assessed by comparing hypovascular and common hypervascular HCC. Methods: The solitary HCCs of 827 patients were classified into two groups according to the tumor density at the arterial phase of a dynamic computed tomography scan; the initial clinical data of patients with the hyper- and hypovascular types were compared. The HCC tissues from 95 tumors were analyzed by immunohistochemical staining for DCP and phosphorylated KDR, and intratumoral microvessel density (MVD) was analyzed to evaluate microvessel angiogenesis. Results: The serum DCP levels (320±3532mAU/mL) and tumor size (18.4±9.0mm) of patients with hypervascular HCC were significantly greater than those with hypovascular HCC (38.7±80mAU/mL and 14.6±5.2mm, P

Original languageEnglish
Pages (from-to)1602-1608
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume27
Issue number10
DOIs
Publication statusPublished - 2012

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Prothrombin
Hepatocellular Carcinoma
Vascular Endothelial Growth Factor Receptor-2
Neoplasms
Microvessels
Human Umbilical Vein Endothelial Cells
Cell Movement
Tomography
Cell Proliferation
Staining and Labeling
Serum

Keywords

  • Des-γ-carboxyl prothrombin
  • Hepatocellular carcinoma
  • Intratumoral microvessel density
  • Kinase insert domain receptor

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Des-γ-carboxyl prothrombin is associated with tumor angiogenesis in hepatocellular carcinoma. / Matsubara, Minoru; Shiraha, Hidenori; Kataoka, Jyunro; Iwamuro, Masaya; Horiguchi, Shigeru; Nishina, Shin Ichi; Takaoka, Nobuyuki; Uemura, Masayuki; Takaki, Akinobu; Nakamura, Shinichiro; Kobayashi, Yoshiyuki; Nouso, Kazuhiro; Yamamoto, Kazuhide.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 27, No. 10, 2012, p. 1602-1608.

Research output: Contribution to journalArticle

Matsubara, Minoru ; Shiraha, Hidenori ; Kataoka, Jyunro ; Iwamuro, Masaya ; Horiguchi, Shigeru ; Nishina, Shin Ichi ; Takaoka, Nobuyuki ; Uemura, Masayuki ; Takaki, Akinobu ; Nakamura, Shinichiro ; Kobayashi, Yoshiyuki ; Nouso, Kazuhiro ; Yamamoto, Kazuhide. / Des-γ-carboxyl prothrombin is associated with tumor angiogenesis in hepatocellular carcinoma. In: Journal of Gastroenterology and Hepatology (Australia). 2012 ; Vol. 27, No. 10. pp. 1602-1608.
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AU - Horiguchi, Shigeru

AU - Nishina, Shin Ichi

AU - Takaoka, Nobuyuki

AU - Uemura, Masayuki

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AU - Nouso, Kazuhiro

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