Derivatives of Dictyostelium discoideum differentiation-inducing factor-3 suppress the activities of Trypanosoma cruzi in vitro and in vivo

Junko Nakajima-Shimada, Toshimitsu Hatabu, Yukari Hosoi, Yoko Onizuka, Haruhisa Kikuchi, Yoshiteru Oshima, Yuzuru Kubohara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chagas disease (human American trypanosomiasis), which is caused by the protozoan parasite Trypanosoma cruzi, is responsible for numerous deaths each year; however, established treatments for the disease are limited. Differentiation-inducing factor-1 (DIF-1) and DIF-3 are chlorinated alkylphenones originally found in the cellular slime mold Dictyostelium discoideum that have been shown to possess pharmacological activities. Here, we investigated the effects of DIF-3 derivatives on the infection rate and growth of T. cruzi by using an in vitro assay system utilizing host human fibrosarcoma HT1080 cells. Certain DIF-3 derivatives, such as butoxy-DIF-3 (Bu-DIF-3), at micro-molar levels strongly suppressed both the infection rate and growth of T. cruzi in HT1080 cells and exhibited little toxicity for HT1080 cells. For example, the IC50 of DIF-3 and Bu-DIF-3 versus the growth of T. cruzi in HT1080 cells were 3.95 and 0.72 μM, respectively, and the LD50 of the two compounds versus HT1080 cells were both greater than 100 μM. We also examined the effects of DIF-3 and Bu-DIF-3 on T. cruzi activity in C57BL/6 mice. Intraperitoneally administered Bu-DIF-3 (50 mg/kg) significantly suppressed the number of trypomastigotes in blood with no apparent adverse effects. These results strongly suggest that DIF-3 derivatives could be new lead compounds in the development of anti-trypanosomiasis drugs.

Original languageEnglish
Pages (from-to)1603-1610
Number of pages8
JournalBiochemical Pharmacology
Volume85
Issue number11
DOIs
Publication statusPublished - Jun 1 2013

Fingerprint

Dictyostelium
Trypanosoma cruzi
Derivatives
Chagas Disease
Lead compounds
Fungi
Growth
Dictyosteliida
Toxicity
Assays
Blood
Trypanosomiasis
Fibrosarcoma
Lethal Dose 50
Infection
Inbred C57BL Mouse
Inhibitory Concentration 50
Parasites
Pharmaceutical Preparations
In Vitro Techniques

Keywords

  • Chagas disease
  • Dictyostelium discoideum
  • DIF-1
  • DIF-3
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Pharmacology
  • Biochemistry

Cite this

Derivatives of Dictyostelium discoideum differentiation-inducing factor-3 suppress the activities of Trypanosoma cruzi in vitro and in vivo. / Nakajima-Shimada, Junko; Hatabu, Toshimitsu; Hosoi, Yukari; Onizuka, Yoko; Kikuchi, Haruhisa; Oshima, Yoshiteru; Kubohara, Yuzuru.

In: Biochemical Pharmacology, Vol. 85, No. 11, 01.06.2013, p. 1603-1610.

Research output: Contribution to journalArticle

Nakajima-Shimada, Junko ; Hatabu, Toshimitsu ; Hosoi, Yukari ; Onizuka, Yoko ; Kikuchi, Haruhisa ; Oshima, Yoshiteru ; Kubohara, Yuzuru. / Derivatives of Dictyostelium discoideum differentiation-inducing factor-3 suppress the activities of Trypanosoma cruzi in vitro and in vivo. In: Biochemical Pharmacology. 2013 ; Vol. 85, No. 11. pp. 1603-1610.
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