Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin

Takuro Masaka, Masahiro Miyazaki, Gang Du, Marhaen Hardjo, Masakiyo Sakaguchi, Mikiro Takaishi, Ken Kataoka, Kazuhide Yamamoto, Nam Ho Huh

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

We have recently established a clonal mesenchymal stem cell line (rBM25/ S3) from adult rat bone marrow. The cells have practically unlimited proliferation capacity (over 300 PDL), maintaining multipotency for differentiation. In the present study, we examined the potential for rBM25/S3 cells to differentiate into insulin-secreting cells. When cultured in the presence of HGF and FGF-4 on Matrigel, rBM25/S3 cells expressed genes specific to pancreatic ß-cells as well as those specific to hepatocytes. They still maintained proliferation capacity with a doubling time of ∼30 h. These hepato-pancreatic intermediate progenitor cells, but not the original undifferentiated rBM25/S3 cells, were induced by the overexpression of PDX-1 to produce significant amounts of insulin in a manner responding to glucose concentration in medium. The present culture system indicates a direction for further studies aimed at the realization of cell transplantation therapy for type I diabetes mellitus.

Original languageEnglish
Pages (from-to)447-452
Number of pages6
JournalInternational journal of molecular medicine
Volume22
Issue number4
DOIs
Publication statusPublished - Oct 2008

Keywords

  • Albumin
  • Insulin
  • Mesenchymal stem cells
  • PDX-1
  • Progenitor cells

ASJC Scopus subject areas

  • Genetics

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