Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin

Takuro Masaka, Masahiro Miyazaki, Gang Du, Marhaen Hardjo, Masakiyo Sakaguchi, Mikiro Takaishi, Ken Kataoka, Kazuhide Yamamoto, Nam Ho Huh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have recently established a clonal mesenchymal stem cell line (rBM25/ S3) from adult rat bone marrow. The cells have practically unlimited proliferation capacity (over 300 PDL), maintaining multipotency for differentiation. In the present study, we examined the potential for rBM25/S3 cells to differentiate into insulin-secreting cells. When cultured in the presence of HGF and FGF-4 on Matrigel, rBM25/S3 cells expressed genes specific to pancreatic ß-cells as well as those specific to hepatocytes. They still maintained proliferation capacity with a doubling time of ∼30 h. These hepato-pancreatic intermediate progenitor cells, but not the original undifferentiated rBM25/S3 cells, were induced by the overexpression of PDX-1 to produce significant amounts of insulin in a manner responding to glucose concentration in medium. The present culture system indicates a direction for further studies aimed at the realization of cell transplantation therapy for type I diabetes mellitus.

Original languageEnglish
Pages (from-to)447-452
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume22
Issue number4
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Mesenchymal Stromal Cells
Stem Cells
Bone Marrow
Cell Line
Cell Transplantation
Insulin-Secreting Cells
Cell- and Tissue-Based Therapy
Type 1 Diabetes Mellitus
Hepatocytes
Insulin
Glucose
Genes

Keywords

  • Albumin
  • Insulin
  • Mesenchymal stem cells
  • PDX-1
  • Progenitor cells

ASJC Scopus subject areas

  • Genetics

Cite this

Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin. / Masaka, Takuro; Miyazaki, Masahiro; Du, Gang; Hardjo, Marhaen; Sakaguchi, Masakiyo; Takaishi, Mikiro; Kataoka, Ken; Yamamoto, Kazuhide; Huh, Nam Ho.

In: International Journal of Molecular Medicine, Vol. 22, No. 4, 10.2008, p. 447-452.

Research output: Contribution to journalArticle

Masaka, Takuro ; Miyazaki, Masahiro ; Du, Gang ; Hardjo, Marhaen ; Sakaguchi, Masakiyo ; Takaishi, Mikiro ; Kataoka, Ken ; Yamamoto, Kazuhide ; Huh, Nam Ho. / Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin. In: International Journal of Molecular Medicine. 2008 ; Vol. 22, No. 4. pp. 447-452.
@article{801d68e89e48474eb2a6e1152ab7a0dc,
title = "Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin",
abstract = "We have recently established a clonal mesenchymal stem cell line (rBM25/ S3) from adult rat bone marrow. The cells have practically unlimited proliferation capacity (over 300 PDL), maintaining multipotency for differentiation. In the present study, we examined the potential for rBM25/S3 cells to differentiate into insulin-secreting cells. When cultured in the presence of HGF and FGF-4 on Matrigel, rBM25/S3 cells expressed genes specific to pancreatic {\ss}-cells as well as those specific to hepatocytes. They still maintained proliferation capacity with a doubling time of ∼30 h. These hepato-pancreatic intermediate progenitor cells, but not the original undifferentiated rBM25/S3 cells, were induced by the overexpression of PDX-1 to produce significant amounts of insulin in a manner responding to glucose concentration in medium. The present culture system indicates a direction for further studies aimed at the realization of cell transplantation therapy for type I diabetes mellitus.",
keywords = "Albumin, Insulin, Mesenchymal stem cells, PDX-1, Progenitor cells",
author = "Takuro Masaka and Masahiro Miyazaki and Gang Du and Marhaen Hardjo and Masakiyo Sakaguchi and Mikiro Takaishi and Ken Kataoka and Kazuhide Yamamoto and Huh, {Nam Ho}",
year = "2008",
month = "10",
doi = "10.3892/ijmm_00000041",
language = "English",
volume = "22",
pages = "447--452",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "4",

}

TY - JOUR

T1 - Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin

AU - Masaka, Takuro

AU - Miyazaki, Masahiro

AU - Du, Gang

AU - Hardjo, Marhaen

AU - Sakaguchi, Masakiyo

AU - Takaishi, Mikiro

AU - Kataoka, Ken

AU - Yamamoto, Kazuhide

AU - Huh, Nam Ho

PY - 2008/10

Y1 - 2008/10

N2 - We have recently established a clonal mesenchymal stem cell line (rBM25/ S3) from adult rat bone marrow. The cells have practically unlimited proliferation capacity (over 300 PDL), maintaining multipotency for differentiation. In the present study, we examined the potential for rBM25/S3 cells to differentiate into insulin-secreting cells. When cultured in the presence of HGF and FGF-4 on Matrigel, rBM25/S3 cells expressed genes specific to pancreatic ß-cells as well as those specific to hepatocytes. They still maintained proliferation capacity with a doubling time of ∼30 h. These hepato-pancreatic intermediate progenitor cells, but not the original undifferentiated rBM25/S3 cells, were induced by the overexpression of PDX-1 to produce significant amounts of insulin in a manner responding to glucose concentration in medium. The present culture system indicates a direction for further studies aimed at the realization of cell transplantation therapy for type I diabetes mellitus.

AB - We have recently established a clonal mesenchymal stem cell line (rBM25/ S3) from adult rat bone marrow. The cells have practically unlimited proliferation capacity (over 300 PDL), maintaining multipotency for differentiation. In the present study, we examined the potential for rBM25/S3 cells to differentiate into insulin-secreting cells. When cultured in the presence of HGF and FGF-4 on Matrigel, rBM25/S3 cells expressed genes specific to pancreatic ß-cells as well as those specific to hepatocytes. They still maintained proliferation capacity with a doubling time of ∼30 h. These hepato-pancreatic intermediate progenitor cells, but not the original undifferentiated rBM25/S3 cells, were induced by the overexpression of PDX-1 to produce significant amounts of insulin in a manner responding to glucose concentration in medium. The present culture system indicates a direction for further studies aimed at the realization of cell transplantation therapy for type I diabetes mellitus.

KW - Albumin

KW - Insulin

KW - Mesenchymal stem cells

KW - PDX-1

KW - Progenitor cells

UR - http://www.scopus.com/inward/record.url?scp=54049096702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54049096702&partnerID=8YFLogxK

U2 - 10.3892/ijmm_00000041

DO - 10.3892/ijmm_00000041

M3 - Article

C2 - 18813850

AN - SCOPUS:54049096702

VL - 22

SP - 447

EP - 452

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 4

ER -