Dentin Phosphophoryn Promotes Cellular Migration of Human Dental Pulp Cells

Yoshiyuki Yasuda, Masanobu Izumikawa, Kuniaki Okamoto, Takayuki Tsukuba, Takashi Saito

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Dentin phosphophoryn (DPP) is a dentin sialophosphoprotein gene product that has an RGD motif and repeat sequences of aspartic acid and phosphoserine. To date, the function of DPP in the early stage of reparative dentin formation still remains unclear. The objective of this study was to evaluate the effects of DPP on pulp cell migration and proliferation. DPP promoted cell migration in a concentration-dependent manner, thus increasing it by about 3-fold at 1000 ng/mL compared with the control, but it had no effect on cell proliferation. Dephosphorylated DPP also promoted cell migration, similarly to DPP. However, cell migration was significantly suppressed by the addition of αvβ3 integrin antibody to the medium. Furthermore, porcine DPP-derived RGD peptide, but not its mutant RAD peptide, significantly promoted cell migration. These results indicated that the RGD motif of DPP plays an important role in the migration of human dental pulp cells.

Original languageEnglish
Pages (from-to)575-578
Number of pages4
JournalJournal of Endodontics
Volume34
Issue number5
DOIs
Publication statusPublished - May 2008

Keywords

  • Dental pulp cells
  • RGD motif
  • dentin sialophosphoprotein
  • migration
  • phosphophoryn
  • reparative dentin

ASJC Scopus subject areas

  • Dentistry(all)

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