TY - JOUR
T1 - Dendritic spine formation in response to progesterone synthesized de novo in the developing Purkinje cell in rats
AU - Sakamoto, Hirotaka
AU - Ukena, Kazuyoshi
AU - Tsutsui, Kazuyoshi
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, Culture and Technology Japan (12440233, 12894021, and 13210101 to K.T.). H.S. is supported by a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2002/4/5
Y1 - 2002/4/5
N2 - The cerebellar Purkinje cell (PC) is a typical site for neurosteroid formation. We have demonstrated that this neuron possesses intranuclear receptor for progesterone and actively synthesizes progesterone de novo from cholesterol only during rat neonatal life, when the formation of the cerebellar cortex occurs dramatically. In this study, we therefore analyzed the effect of progesterone on dendritic spine formation of the PC. In vitro studies using cerebellar slice cultures from newborn rats showed that progesterone increases the density of PC dendritic spines in a dose-dependent manner. This effect was blocked by the progesterone receptor antagonist, RU486. Furthermore, trilostane, a specific inhibitor of progesterone synthesis, inhibited the increase of spine density. These results suggest that progesterone can promote dendritic spine formation, and endogenous progesterone synthesized de novo in the developing PC may induce such an effect.
AB - The cerebellar Purkinje cell (PC) is a typical site for neurosteroid formation. We have demonstrated that this neuron possesses intranuclear receptor for progesterone and actively synthesizes progesterone de novo from cholesterol only during rat neonatal life, when the formation of the cerebellar cortex occurs dramatically. In this study, we therefore analyzed the effect of progesterone on dendritic spine formation of the PC. In vitro studies using cerebellar slice cultures from newborn rats showed that progesterone increases the density of PC dendritic spines in a dose-dependent manner. This effect was blocked by the progesterone receptor antagonist, RU486. Furthermore, trilostane, a specific inhibitor of progesterone synthesis, inhibited the increase of spine density. These results suggest that progesterone can promote dendritic spine formation, and endogenous progesterone synthesized de novo in the developing PC may induce such an effect.
KW - 3α,5α-Tetrahydroprogesterone
KW - Cerebellar development
KW - Dendritic spine formation
KW - Neurosteroids
KW - Progesterone
KW - Purkinje cell
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U2 - 10.1016/S0304-3940(02)00077-0
DO - 10.1016/S0304-3940(02)00077-0
M3 - Article
C2 - 11958856
AN - SCOPUS:0037023245
SN - 0304-3940
VL - 322
SP - 111
EP - 115
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -