Dendritic growth in response to environmental estrogens in the developing Purkinje cell in rats

Hanako Shikimi, Hirotaka Sakamoto, Yukio Mezaki, Kazuyoshi Ukena, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The cerebellar Purkinje cell is a major site for neurosteroid formation. We have demonstrated recently that the Purkinje cell actively produces sex steroids, such as estradiol and progesterone, de novo from cholesterol only during rat neonatal life, when cerebellar cortical formation occurs. We have further demonstrated that both estradiol and progesterone promote the growth of Purkinje cells through intranuclear receptor-mediated mechanisms during cerebellar development. On the other hand, environmental estrogens, such as octylphenol (OP), bisphenol A (BPA), and nonylphenol (NP) are thought to mimic the action of estrogen in the developing central nervous system. Therefore, in this study, the effect of these environmental estrogens on the growth of Purkinje cells was examined in vivo using newborn rats. OP and BPA promoted a dose-dependent dendritic outgrowth of the Purkinje cell but did not affect its soma and cell number. The stimulatory effect of OP and BPA on Purkinje dendritic growth was induced by an injection of 500 μg/day into the cerebrospinal fluid for 4 days and blocked by the estrogen receptor antagonist tamoxifen. However, there was no significant effect of NP on any Purkinje cell morphology. These results suggest that the environmental estrogens, OP and BPA, promote Purkinje dendritic growth during neonatal life. This effect may be mediated by estrogen receptor in the Purkinje cell.

Original languageEnglish
Pages (from-to)114-118
Number of pages5
JournalNeuroscience Letters
Volume364
Issue number2
DOIs
Publication statusPublished - Jul 1 2004
Externally publishedYes

Fingerprint

Purkinje Cells
Estrogens
Growth
Progesterone
Estradiol
Carisoprodol
Tamoxifen
Estrogen Receptors
Neurotransmitter Agents
Cerebrospinal Fluid
Central Nervous System
Cell Count
Steroids
Cholesterol
Injections
octylphenol
bisphenol A

Keywords

  • Dendritic growth
  • Environmental estrogens
  • Estradiol
  • Purkinje cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dendritic growth in response to environmental estrogens in the developing Purkinje cell in rats. / Shikimi, Hanako; Sakamoto, Hirotaka; Mezaki, Yukio; Ukena, Kazuyoshi; Tsutsui, Kazuyoshi.

In: Neuroscience Letters, Vol. 364, No. 2, 01.07.2004, p. 114-118.

Research output: Contribution to journalArticle

Shikimi, Hanako ; Sakamoto, Hirotaka ; Mezaki, Yukio ; Ukena, Kazuyoshi ; Tsutsui, Kazuyoshi. / Dendritic growth in response to environmental estrogens in the developing Purkinje cell in rats. In: Neuroscience Letters. 2004 ; Vol. 364, No. 2. pp. 114-118.
@article{4ddbac0a06cc40a19ed9fedc6ae91a53,
title = "Dendritic growth in response to environmental estrogens in the developing Purkinje cell in rats",
abstract = "The cerebellar Purkinje cell is a major site for neurosteroid formation. We have demonstrated recently that the Purkinje cell actively produces sex steroids, such as estradiol and progesterone, de novo from cholesterol only during rat neonatal life, when cerebellar cortical formation occurs. We have further demonstrated that both estradiol and progesterone promote the growth of Purkinje cells through intranuclear receptor-mediated mechanisms during cerebellar development. On the other hand, environmental estrogens, such as octylphenol (OP), bisphenol A (BPA), and nonylphenol (NP) are thought to mimic the action of estrogen in the developing central nervous system. Therefore, in this study, the effect of these environmental estrogens on the growth of Purkinje cells was examined in vivo using newborn rats. OP and BPA promoted a dose-dependent dendritic outgrowth of the Purkinje cell but did not affect its soma and cell number. The stimulatory effect of OP and BPA on Purkinje dendritic growth was induced by an injection of 500 μg/day into the cerebrospinal fluid for 4 days and blocked by the estrogen receptor antagonist tamoxifen. However, there was no significant effect of NP on any Purkinje cell morphology. These results suggest that the environmental estrogens, OP and BPA, promote Purkinje dendritic growth during neonatal life. This effect may be mediated by estrogen receptor in the Purkinje cell.",
keywords = "Dendritic growth, Environmental estrogens, Estradiol, Purkinje cell",
author = "Hanako Shikimi and Hirotaka Sakamoto and Yukio Mezaki and Kazuyoshi Ukena and Kazuyoshi Tsutsui",
year = "2004",
month = "7",
day = "1",
doi = "10.1016/j.neulet.2004.04.023",
language = "English",
volume = "364",
pages = "114--118",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Dendritic growth in response to environmental estrogens in the developing Purkinje cell in rats

AU - Shikimi, Hanako

AU - Sakamoto, Hirotaka

AU - Mezaki, Yukio

AU - Ukena, Kazuyoshi

AU - Tsutsui, Kazuyoshi

PY - 2004/7/1

Y1 - 2004/7/1

N2 - The cerebellar Purkinje cell is a major site for neurosteroid formation. We have demonstrated recently that the Purkinje cell actively produces sex steroids, such as estradiol and progesterone, de novo from cholesterol only during rat neonatal life, when cerebellar cortical formation occurs. We have further demonstrated that both estradiol and progesterone promote the growth of Purkinje cells through intranuclear receptor-mediated mechanisms during cerebellar development. On the other hand, environmental estrogens, such as octylphenol (OP), bisphenol A (BPA), and nonylphenol (NP) are thought to mimic the action of estrogen in the developing central nervous system. Therefore, in this study, the effect of these environmental estrogens on the growth of Purkinje cells was examined in vivo using newborn rats. OP and BPA promoted a dose-dependent dendritic outgrowth of the Purkinje cell but did not affect its soma and cell number. The stimulatory effect of OP and BPA on Purkinje dendritic growth was induced by an injection of 500 μg/day into the cerebrospinal fluid for 4 days and blocked by the estrogen receptor antagonist tamoxifen. However, there was no significant effect of NP on any Purkinje cell morphology. These results suggest that the environmental estrogens, OP and BPA, promote Purkinje dendritic growth during neonatal life. This effect may be mediated by estrogen receptor in the Purkinje cell.

AB - The cerebellar Purkinje cell is a major site for neurosteroid formation. We have demonstrated recently that the Purkinje cell actively produces sex steroids, such as estradiol and progesterone, de novo from cholesterol only during rat neonatal life, when cerebellar cortical formation occurs. We have further demonstrated that both estradiol and progesterone promote the growth of Purkinje cells through intranuclear receptor-mediated mechanisms during cerebellar development. On the other hand, environmental estrogens, such as octylphenol (OP), bisphenol A (BPA), and nonylphenol (NP) are thought to mimic the action of estrogen in the developing central nervous system. Therefore, in this study, the effect of these environmental estrogens on the growth of Purkinje cells was examined in vivo using newborn rats. OP and BPA promoted a dose-dependent dendritic outgrowth of the Purkinje cell but did not affect its soma and cell number. The stimulatory effect of OP and BPA on Purkinje dendritic growth was induced by an injection of 500 μg/day into the cerebrospinal fluid for 4 days and blocked by the estrogen receptor antagonist tamoxifen. However, there was no significant effect of NP on any Purkinje cell morphology. These results suggest that the environmental estrogens, OP and BPA, promote Purkinje dendritic growth during neonatal life. This effect may be mediated by estrogen receptor in the Purkinje cell.

KW - Dendritic growth

KW - Environmental estrogens

KW - Estradiol

KW - Purkinje cell

UR - http://www.scopus.com/inward/record.url?scp=2942652879&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942652879&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2004.04.023

DO - 10.1016/j.neulet.2004.04.023

M3 - Article

VL - 364

SP - 114

EP - 118

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2

ER -