Dendritic growth and spine formation in response to estrogen in the developing Purkinje cell

Hirotaka Sakamoto, Yukio Mezaki, Hanako Shikimi, Kazuyoshi Ukena, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

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Abstract

Neurosteroids are synthesized de novo in the brain, and the cerebellar Purkinje cell is a major site for neurosteroid formation. We have demonstrated that the Purkinje cell possesses intranuclear receptor for progesterone and actively produces progesterone de novo from cholesterol only during rat neonatal life, when cerebellar cortical formation occurs dramatically. We have further demonstrated that progesterone promotes dendritic growth, spinogenesis, and synaptogenesis via its receptor in this neuron in the neonate. On the other hand, estrogen may also play an important role in the process of cerebellar cortical formation, because the neonatal rat Purkinje cell possesses estrogen receptor (ER)β. However, estrogen formation in the neonatal cerebellum is still unclear. In this study, we therefore analyzed the biosynthesis and action of estrogen in Purkinje cells during neonatal life. RT-PCR-Southern and in situ hybridization analyses showed that Purkinje cells expressed the key enzyme of estrogen formation, cytochrome P450 aromatase, in neonatal rats. A specific enzyme immunoassay for estradiol further indicated that cerebellar estradiol concentrations in the neonate were significantly higher than those in the prepuberty and adult. Both in vitro and in vivo studies with newborn rats showed that estradiol promoted dose-dependent dendritic growth of Purkinje cells. Estradiol also increased the density of Purkinje dendritic spines. These effects were inhibited by the ER antagonist tamoxifen. These results suggest that estradiol in the developing Purkinje cell promotes dendritic growth and spinogenesis via ERβ in this neuron. Estradiol as well as progesterone may contribute to the growth of Purkinje cells during the cerebellar cortical formation.

Original languageEnglish
Pages (from-to)4466-4477
Number of pages12
JournalEndocrinology
Volume144
Issue number10
DOIs
Publication statusPublished - Oct 1 2003
Externally publishedYes

Fingerprint

Dendritic Spines
Purkinje Cells
Estrogens
Estradiol
Growth
Progesterone
Estrogen Receptors
Neurotransmitter Agents
Neurons
Aromatase
Progesterone Receptors
Tamoxifen
Immunoenzyme Techniques
Cytochrome P-450 Enzyme System
Cerebellum
In Situ Hybridization
Cholesterol
Polymerase Chain Reaction
Brain
Enzymes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Dendritic growth and spine formation in response to estrogen in the developing Purkinje cell. / Sakamoto, Hirotaka; Mezaki, Yukio; Shikimi, Hanako; Ukena, Kazuyoshi; Tsutsui, Kazuyoshi.

In: Endocrinology, Vol. 144, No. 10, 01.10.2003, p. 4466-4477.

Research output: Contribution to journalArticle

Sakamoto, Hirotaka ; Mezaki, Yukio ; Shikimi, Hanako ; Ukena, Kazuyoshi ; Tsutsui, Kazuyoshi. / Dendritic growth and spine formation in response to estrogen in the developing Purkinje cell. In: Endocrinology. 2003 ; Vol. 144, No. 10. pp. 4466-4477.
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