Dendritic cell subsets and immunological milieu in inflammatory human papilloma virus-related skin lesions

Yumi Nakayama, Kenji Asagoe, Akiko Yamauchi, Takenobu Yamamoto, Yoshinori Shirafuji, Shin Morizane, Gen Nakanishi, Keiji Iwatsuki

Research output: Contribution to journalArticle

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Abstract

Background: Human papilloma virus (HPV)-related warts persist, evading host immune surveillance, but sometimes disappear with inflammation. Objectives: To elucidate the immune evasion mechanisms of HPV, we have examined the density, dynamics, and subsets of dendritic cell (DC) types in non-inflammatory or inflammatory HPV-related skin lesions such as warts and Bowen's disease (HPV-Bowen), and compared the epidermal expression levels of macrophage inflammatory protein (MIP)-3α and E-cadherin. Methods: The expression of various DC markers, MIP-3α, and E-cadherin in the tissue samples obtained from patients with warts, HPV-Bowen and HPV-unrelated skin diseases was evaluated by immunohistochemistry. MIP-3α gene expression levels were examined in warts and HPV-Bowen by in situ hybridization (ISH) and real-time quantitative polymerase chain reaction (RT-qPCR). Results: The numbers of Langerhans cells (LCs) and the expression levels of MIP-3α and E-cadherin were decreased in non-inflammatory warts and HPV-Bowen, as compared with normal skin. Both epidermal LCs and MIP-3α expression reappeared in inflammatory warts, associated with dermal infiltrates composed of many cytotoxic T cells and various subsets of DCs, while cellular infiltrates in HPV-Bowen contained many B cells and plasma cells with sparse infiltration of DCs. The upregulation of MIP-3α gene expression was confirmed in the inflammatory warts and HPV-Bowen by ISH and RT-qPCR. Conclusions: The depletion of LCs in the non-inflammatory warts and HPV-Bowen is associated with a down-regulation of expression levels of MIP-3α and E-cadherin in the lesional keratinocytes. MIP-3α expression is upregulated in lesional keratinocytes of inflammatory warts, with the subsequent recruitment of various DC subsets and cytotoxic T cells, whereas plasma cell-rich infiltration was induced in HPV-Bowen.

Original languageEnglish
Pages (from-to)173-183
Number of pages11
JournalJournal of Dermatological Science
Volume63
Issue number3
DOIs
Publication statusPublished - Sep 2011

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Papillomaviridae
Viruses
Dendritic Cells
Warts
Macrophage Inflammatory Proteins
Skin
Cadherins
Langerhans Cells
T-cells
Polymerase chain reaction
T-Lymphocyte Subsets
Plasma Cells
Keratinocytes
Infiltration
Gene expression
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Bowen's Disease
Immune Evasion
Plasmas

Keywords

  • Dendritic cell
  • E-cadherin
  • Human papilloma virus
  • Langerhans cell
  • MIP-3α

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Molecular Biology

Cite this

Dendritic cell subsets and immunological milieu in inflammatory human papilloma virus-related skin lesions. / Nakayama, Yumi; Asagoe, Kenji; Yamauchi, Akiko; Yamamoto, Takenobu; Shirafuji, Yoshinori; Morizane, Shin; Nakanishi, Gen; Iwatsuki, Keiji.

In: Journal of Dermatological Science, Vol. 63, No. 3, 09.2011, p. 173-183.

Research output: Contribution to journalArticle

Nakayama, Y, Asagoe, K, Yamauchi, A, Yamamoto, T, Shirafuji, Y, Morizane, S, Nakanishi, G & Iwatsuki, K 2011, 'Dendritic cell subsets and immunological milieu in inflammatory human papilloma virus-related skin lesions', Journal of Dermatological Science, vol. 63, no. 3, pp. 173-183. https://doi.org/10.1016/j.jdermsci.2011.05.006
Nakayama, Yumi ; Asagoe, Kenji ; Yamauchi, Akiko ; Yamamoto, Takenobu ; Shirafuji, Yoshinori ; Morizane, Shin ; Nakanishi, Gen ; Iwatsuki, Keiji. / Dendritic cell subsets and immunological milieu in inflammatory human papilloma virus-related skin lesions. In: Journal of Dermatological Science. 2011 ; Vol. 63, No. 3. pp. 173-183.
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