Demonstration of receptors for parathyroid hormone on cultured rabbit costal chondrocytes

Motomi Enomoto, Akihiro Kinoshita, Hai Ou Pan, Fujio Suzuki, Itsuo Yamamoto, Masaharu Takigawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Parathyroid hormone (PTH) receptors on cultured rabbit costal chondrocytes were demonstrated using HPLC-purified, radioiodinated [Nle8,-Nle18,Tyr34]bovine PTH-(1-34)amide. PTH binding was found to be specific for PTH agonists and antagonists and dependent on the time and temperature of incubation. Both growth cartilage (GC) cells and resting cartilage (RC) cells were shown to have a single class of saturable, high affinity PTH binding sites with a dissociation constant of 0.6-0.7 nM. However, the numbers of receptors per cell were approximately 49,000 on GC cells and 19,000 on RC cells. After crosslinking the receptors on these cells with the radioligand, one, major 125I-labeled band of 76 kDa was separated by SDS-PAGE.

Original languageEnglish
Pages (from-to)1222-1229
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume162
Issue number3
DOIs
Publication statusPublished - Aug 15 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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