TY - JOUR
T1 - Demonstration of receptors for epidermal growth factor on cultured rabbit chondrocytes and regulation of their expression by various growth and differentiation factors
AU - Kinoshita, Akihiro
AU - Takigawa, Masaharu
AU - Suzuki, Fujio
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research and Developmental Scientific Research from the Ministry of Education, Science and Culture of Japan (M.T. and F.S.) , and grants from the Takeda Science Foundation (M.T.) and the Naito Foundation (M.T.). We thank Mrs.Elizabeth lchihara for assistance in the preparation of this manuscript and Miss Noriko Yoneda for her secretarial assistance.
PY - 1992/2/28
Y1 - 1992/2/28
N2 - Epidermal growth factor (EGF) receptors were demonstrated on cultured rabbit costal chondrocytes. After crosslinking, the receptors on the cells with 125I-EGF, one major band of 170 KDa was separated by SDS-PAGE. Scatchard analysis demonstrated two classes of EGF receptors with Kd values of 0.3 nM and 1.6 nM. The numbers of high and low affinity receptors were 3,000 and 10,000 per cell, respectively. EGF receptors on chondrocytes were increased by treatment with retinoic acid and interleukin-1β, which inhibited proteoglycan synthesis. On the other hand, parathyroid hormone and dibutyryl cyclic AMP, which stimulated proteoglycan synthesis, decreased the number of EGF receptors. Treatments with these agents did not change the affinity of the receptors. These findings suggest that the number of EGF receptors is a negative marker of chondrocyte differentiation.
AB - Epidermal growth factor (EGF) receptors were demonstrated on cultured rabbit costal chondrocytes. After crosslinking, the receptors on the cells with 125I-EGF, one major band of 170 KDa was separated by SDS-PAGE. Scatchard analysis demonstrated two classes of EGF receptors with Kd values of 0.3 nM and 1.6 nM. The numbers of high and low affinity receptors were 3,000 and 10,000 per cell, respectively. EGF receptors on chondrocytes were increased by treatment with retinoic acid and interleukin-1β, which inhibited proteoglycan synthesis. On the other hand, parathyroid hormone and dibutyryl cyclic AMP, which stimulated proteoglycan synthesis, decreased the number of EGF receptors. Treatments with these agents did not change the affinity of the receptors. These findings suggest that the number of EGF receptors is a negative marker of chondrocyte differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0026537977&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026537977&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(92)91601-L
DO - 10.1016/0006-291X(92)91601-L
M3 - Article
C2 - 1311919
AN - SCOPUS:0026537977
VL - 183
SP - 14
EP - 20
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -