Demonstration of endothelin (ET) receptors on cultured rabbit chondrocytes and stimulation of DNA synthesis and calcium influx by ET-1 via its receptors

Akihiro Kinoshita, Tomoo Tamura, Chiharu Sogawa, Tohru Nakanishi, Shizuo Sobue, Fujio Suzuki, Kojiro Takahashi, Masaharu Takigawa

Research output: Contribution to journalArticle

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Abstract

Endothelin (ET) receptors on chondrocytes were demonstrated using cultured rabbit costal chondrocytes. After crosslinking the receptors on the cells with 125 I-ET-1, two major bands of 43 kDa and 46 kDa were separated by SDS-PAGE. Scatchard analysis demonstrated two classes of ET receptors with Kd values of 1 × 10-10 M and 5 × 10-9 M. The numbers of high- and low- affinity receptors were 1 × 104 and 2 × 105 per cell, respectively. The binding of ET-1 to chondrocytes was increased by treatment with PTH, DBcAMP, TGF-β1, IL-1β, RA and EGF. ET-1 stimulated DNA synthesis in cultured rabbit chondrocytes. ET-1 also stimulated calcium incorporation through the cell membrane of chondrocytes. These findings indicate that ET-1 has a physiological effect on chondrocytes via its receptors on the cells.

Original languageEnglish
Pages (from-to)647-654
Number of pages8
JournalCell Biology International
Volume19
Issue number8
DOIs
Publication statusPublished - Aug 1995

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Endothelin Receptors
Endothelin-1
Chondrocytes
Rabbits
Calcium
DNA
Bucladesine
Interleukin-1
Epidermal Growth Factor
Polyacrylamide Gel Electrophoresis
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology

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Demonstration of endothelin (ET) receptors on cultured rabbit chondrocytes and stimulation of DNA synthesis and calcium influx by ET-1 via its receptors. / Kinoshita, Akihiro; Tamura, Tomoo; Sogawa, Chiharu; Nakanishi, Tohru; Sobue, Shizuo; Suzuki, Fujio; Takahashi, Kojiro; Takigawa, Masaharu.

In: Cell Biology International, Vol. 19, No. 8, 08.1995, p. 647-654.

Research output: Contribution to journalArticle

Kinoshita, Akihiro ; Tamura, Tomoo ; Sogawa, Chiharu ; Nakanishi, Tohru ; Sobue, Shizuo ; Suzuki, Fujio ; Takahashi, Kojiro ; Takigawa, Masaharu. / Demonstration of endothelin (ET) receptors on cultured rabbit chondrocytes and stimulation of DNA synthesis and calcium influx by ET-1 via its receptors. In: Cell Biology International. 1995 ; Vol. 19, No. 8. pp. 647-654.
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