TY - JOUR
T1 - Demonstration of endothelin (ET) receptors on cultured rabbit chondrocytes and stimulation of DNA synthesis and calcium influx by ET-1 via its receptors
AU - Kinoshita, Akihiro
AU - Tamura, Tomoo
AU - Sogawa, Chiharu
AU - Nakanishi, Tohru
AU - Sobue, Shizuo
AU - Suzuki, Fujio
AU - Takahashi, Kojiro
AU - Takigawa, Masaharu
PY - 1995/8
Y1 - 1995/8
N2 - Endothelin (ET) receptors on chondrocytes were demonstrated using cultured rabbit costal chondrocytes. After crosslinking the receptors on the cells with 125 I-ET-1, two major bands of 43 kDa and 46 kDa were separated by SDS-PAGE. Scatchard analysis demonstrated two classes of ET receptors with Kd values of 1 × 10-10 M and 5 × 10-9 M. The numbers of high- and low- affinity receptors were 1 × 104 and 2 × 105 per cell, respectively. The binding of ET-1 to chondrocytes was increased by treatment with PTH, DBcAMP, TGF-β1, IL-1β, RA and EGF. ET-1 stimulated DNA synthesis in cultured rabbit chondrocytes. ET-1 also stimulated calcium incorporation through the cell membrane of chondrocytes. These findings indicate that ET-1 has a physiological effect on chondrocytes via its receptors on the cells.
AB - Endothelin (ET) receptors on chondrocytes were demonstrated using cultured rabbit costal chondrocytes. After crosslinking the receptors on the cells with 125 I-ET-1, two major bands of 43 kDa and 46 kDa were separated by SDS-PAGE. Scatchard analysis demonstrated two classes of ET receptors with Kd values of 1 × 10-10 M and 5 × 10-9 M. The numbers of high- and low- affinity receptors were 1 × 104 and 2 × 105 per cell, respectively. The binding of ET-1 to chondrocytes was increased by treatment with PTH, DBcAMP, TGF-β1, IL-1β, RA and EGF. ET-1 stimulated DNA synthesis in cultured rabbit chondrocytes. ET-1 also stimulated calcium incorporation through the cell membrane of chondrocytes. These findings indicate that ET-1 has a physiological effect on chondrocytes via its receptors on the cells.
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U2 - 10.1006/cbir.1995.1114
DO - 10.1006/cbir.1995.1114
M3 - Article
C2 - 7550073
AN - SCOPUS:0029119587
SN - 1065-6995
VL - 19
SP - 647
EP - 654
JO - Cell Biology International Reports
JF - Cell Biology International Reports
IS - 8
ER -