Demonstration of endothelin (ET) receptors on cultured rabbit chondrocytes and stimulation of DNA synthesis and calcium influx by ET-1 via its receptors

Akihiro Kinoshita, Tomoo Tamura, Chiharu Aoki, Tohru Nakanishi, Shizuo Sobue, Fujio Suzuki, Kojiro Takahashi, Masaharu Takigawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Endothelin (ET) receptors on chondrocytes were demonstrated using cultured rabbit costal chondrocytes. After crosslinking the receptors on the cells with 125 I-ET-1, two major bands of 43 kDa and 46 kDa were separated by SDS-PAGE. Scatchard analysis demonstrated two classes of ET receptors with Kd values of 1 × 10-10 M and 5 × 10-9 M. The numbers of high- and low- affinity receptors were 1 × 104 and 2 × 105 per cell, respectively. The binding of ET-1 to chondrocytes was increased by treatment with PTH, DBcAMP, TGF-β1, IL-1β, RA and EGF. ET-1 stimulated DNA synthesis in cultured rabbit chondrocytes. ET-1 also stimulated calcium incorporation through the cell membrane of chondrocytes. These findings indicate that ET-1 has a physiological effect on chondrocytes via its receptors on the cells.

Original languageEnglish
Pages (from-to)647-654
Number of pages8
JournalCell Biology International
Volume19
Issue number8
DOIs
Publication statusPublished - Aug 1995

ASJC Scopus subject areas

  • Cell Biology

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