Demonstration of a Light-Driven SO4 2- Transporter and Its Spectroscopic Characteristics

Akiko Niho, Susumu Yoshizawa, Takashi Tsukamoto, Marie Kurihara, Shinya Tahara, Yu Nakajima, Misao Mizuno, Hikaru Kuramochi, Tahei Tahara, Yasuhisa Mizutani, Yuki Sudo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In organisms, ion transporters play essential roles in the generation and dissipation of ion gradients across cell membranes. Microbial rhodopsins selectively transport cognate ions using solar energy, in which the substrate ions identified to date have been confined to monovalent ions such as H+, Na+, and Cl-. Here we report a novel rhodopsin from the cyanobacterium Synechocystis sp. PCC 7509, which inwardly transports a polyatomic divalent sulfate ion, SO4 2-, with changes of its spectroscopic properties in both unphotolyzed and photolyzed states. Upon illumination, cells expressing the novel rhodopsin, named Synechocystis halorhodopsin (SyHR), showed alkalization of the medium only in the presence of Cl- or SO4 2-. That alkalization signal was enhanced by addition of a protonophore, indicating an inward transport of Cl- and SO4 2- with a subsequent secondary inward H+ movement across the membrane. The anion binding to SyHR was suggested by absorption spectral shifts from 542 to 536 nm for Cl- and from 542 to 556 nm for SO4 2-, and the affinities of Cl- and SO4 2- were estimated as 0.112 and 5.81 mM, respectively. We then performed time-resolved spectroscopic measurements ranging from femtosecond to millisecond time domains to elucidate the structure and structural changes of SyHR during the photoreaction. Based on the results, we propose a photocycle model for SyHR in the absence or presence of substrate ions with the timing of their uptake and release. Thus, we demonstrate SyHR as the first light-driven polyatomic divalent anion (SO4 2-) transporter and report its spectroscopic characteristics.

Original languageEnglish
Pages (from-to)4376-4389
Number of pages14
JournalJournal of the American Chemical Society
Volume139
Issue number12
DOIs
Publication statusPublished - Mar 29 2017

Fingerprint

Halorhodopsins
Synechocystis
Demonstrations
Ions
Light
Rhodopsin
Anions
Microbial Rhodopsins
Solar Energy
Negative ions
Ion Transport
Cyanobacteria
Lighting
Sulfates
Substrates
Cell membranes
Solar energy
Cell Membrane
Cells
Membranes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Demonstration of a Light-Driven SO4 2- Transporter and Its Spectroscopic Characteristics. / Niho, Akiko; Yoshizawa, Susumu; Tsukamoto, Takashi; Kurihara, Marie; Tahara, Shinya; Nakajima, Yu; Mizuno, Misao; Kuramochi, Hikaru; Tahara, Tahei; Mizutani, Yasuhisa; Sudo, Yuki.

In: Journal of the American Chemical Society, Vol. 139, No. 12, 29.03.2017, p. 4376-4389.

Research output: Contribution to journalArticle

Niho, A, Yoshizawa, S, Tsukamoto, T, Kurihara, M, Tahara, S, Nakajima, Y, Mizuno, M, Kuramochi, H, Tahara, T, Mizutani, Y & Sudo, Y 2017, 'Demonstration of a Light-Driven SO4 2- Transporter and Its Spectroscopic Characteristics', Journal of the American Chemical Society, vol. 139, no. 12, pp. 4376-4389. https://doi.org/10.1021/jacs.6b12139
Niho, Akiko ; Yoshizawa, Susumu ; Tsukamoto, Takashi ; Kurihara, Marie ; Tahara, Shinya ; Nakajima, Yu ; Mizuno, Misao ; Kuramochi, Hikaru ; Tahara, Tahei ; Mizutani, Yasuhisa ; Sudo, Yuki. / Demonstration of a Light-Driven SO4 2- Transporter and Its Spectroscopic Characteristics. In: Journal of the American Chemical Society. 2017 ; Vol. 139, No. 12. pp. 4376-4389.
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