Demonstration of a ferric vibrioferrin-binding protein in the outer membrane of Vibrio parahaemolyticus

S. Yamamoto, T. Akiyama, N. Okujo, S. Matsu-ura, S. Shinoda

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18 Citations (Scopus)

Abstract

Under iron-restricted conditions, Vibrio parahaemolyticus produces a siderophore, vibrioferrin, accompanying expression of two major outer membrane proteins of 78 and 83 kDa. Autoradiographic analysis of nondenaturing polyacrylamide gel electrophoregrams of outer membrane preparations previously incubated with [55Fe]ferric vibrioferrin revealed a single radiolabeled band, in which the 78-kDa protein was detected predominantly by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antiserum against the purified 78-kDa protein partially inhibited Fe-VF binding to isolated OMPs. The 78-kDa protein was cleaved by the treatment of whole cells with proteinase K, indicating that a portion of this protein is exposed on the surface of the outer membrane. The treated cells lost most of their iron uptake activity mediated by vibrioferrin. These results suggest that the ferric vibrioferrin-binding protein of 78 kDa may function as the receptor for ferric vibrioferrin involved in the initial step of vibrioferrin-mediated iron uptake. Immunoblot analysis using the antiserum against the 78-kDa protein demonstrated that the molecular mass and antigenic properties of the protein were highly conserved among V. parahaemolyticus strains examined. The antiserum also recognized an iron-repressible outer membrane protein of 78 kDa from iron-restricted V. alginolyticus strains, some of which appeared to produce vibrioferrin.

Original languageEnglish
Pages (from-to)759-766
Number of pages8
JournalMicrobiology and Immunology
Volume39
Issue number10
Publication statusPublished - 1995

Keywords

  • Ferric vibrioferrin
  • Iron transport
  • Outer membrane protein
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

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    Yamamoto, S., Akiyama, T., Okujo, N., Matsu-ura, S., & Shinoda, S. (1995). Demonstration of a ferric vibrioferrin-binding protein in the outer membrane of Vibrio parahaemolyticus. Microbiology and Immunology, 39(10), 759-766.