Delivery of sodium borocaptate to glioma cells using immunoliposome conjugated with anti-EGFR antibodies by ZZ-His

Bin Feng, Kazuhito Tomizawa, Hiroyuki Michiue, Shin ichi Miyatake, Xiao Jian Han, Atsushi Fujimura, Masaharu Seno, Mitsunori Kirihata, Hideki Matsui

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Nanoparticles are effective of delivering cargo into cells. Here, sodium borocaptate (BSH) was encapsulated in liposomes composed of nickel lipid, and anti-epidermal growth factor receptor (EGFR) antibodies were conjugated to the liposomes using the antibody affinity motif of protein A (ZZ) as an adaptor (immunoliposomes). The immunoliposomes were used to deliver BSH into EGFR-overexpressing glioma cells. Immunohistochemical analysis using an anti-BSH monoclonal antibody revealed that BSH was delivered effectively into the cells but not into EGFR-deficient glioma or primary astrocytes. In an animal model of brain tumors, both the liposomes and the BSH were only observed in the tumor. Moreover, the efficiency of 10B's delivery into glioma cells was confirmed by inductively coupled plasma-atomic emission spectrometry (ICP-AES) both in vitro and in vivo. The results suggest that this system utilizing immunoliposomes provides an effective means of delivering 10B into glioma cells in boron neutron capture therapy (BNCT).

Original languageEnglish
Pages (from-to)1746-1755
Number of pages10
JournalBiomaterials
Volume30
Issue number9
DOIs
Publication statusPublished - Mar 2009

Fingerprint

Liposomes
Epidermal Growth Factor Receptor
Antibodies
Glioma
Sodium
Tumors
Boron
Monoclonal antibodies
Staphylococcal Protein A
Inductively coupled plasma
Nickel
Lipids
Spectrometry
Boron Neutron Capture Therapy
Brain
Neutrons
Animals
Monoclonal Antibodies
Antibody Affinity
Nanoparticles

Keywords

  • BNCT
  • Drug delivery
  • EGFR
  • Glioma cells
  • Immunoliposome

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Delivery of sodium borocaptate to glioma cells using immunoliposome conjugated with anti-EGFR antibodies by ZZ-His. / Feng, Bin; Tomizawa, Kazuhito; Michiue, Hiroyuki; Miyatake, Shin ichi; Han, Xiao Jian; Fujimura, Atsushi; Seno, Masaharu; Kirihata, Mitsunori; Matsui, Hideki.

In: Biomaterials, Vol. 30, No. 9, 03.2009, p. 1746-1755.

Research output: Contribution to journalArticle

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