Delivery of dsRNA with lactic acid bacteria for RNA interference.

A. Kuwahara, Masashi Arita, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

RNA interference (RNAi) mechanism is promising for gene-targeted therapy. In this study, Lactobacillus casei were engineered to produce long double-stranded RNA (dsRNA) or short hairpin RNA (shRNA) for RNAi-delivery to the intestine. We prepared two kinds of L. casei; one produces shRNA which works in mammalian cells, and the other produces long dsRNA which works in nematode. We confirmed the expression of shRNA and dsRNA in the transformed L. casei by Northern blotting and RT-PCR analyses.

Original languageEnglish
Pages (from-to)413-414
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007

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Lactobacillus casei
Double-Stranded RNA
RNA Interference
Small Interfering RNA
Lactic Acid
Bacteria
Northern Blotting
Genetic Therapy
Intestines
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Delivery of dsRNA with lactic acid bacteria for RNA interference. / Kuwahara, A.; Arita, Masashi; Sisido, Masahiko; Ohtsuki, Takashi.

In: Nucleic acids symposium series (2004), No. 51, 2007, p. 413-414.

Research output: Contribution to journalArticle

Kuwahara, A. ; Arita, Masashi ; Sisido, Masahiko ; Ohtsuki, Takashi. / Delivery of dsRNA with lactic acid bacteria for RNA interference. In: Nucleic acids symposium series (2004). 2007 ; No. 51. pp. 413-414.
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