Delineation of three functional domains of the transcriptional activator encoded by the c-myb protooncogene

H. Sakura, C. Kanei-Ishii, T. Nagase, H. Nakagoshi, T. J. Gonda, S. Ishii

Research output: Contribution to journalArticle

270 Citations (Scopus)

Abstract

The c-myb protooncogene encodes a sequence-specific DNA-binding protein (c-Myb) that induces transcriptional activation or repression. We have identified three functional domains of the mouse c-Myb protein that are responsible for DNA binding, transcriptional activation, and negative regulation, respectively. In addition to the DNA-binding domain, which is located near the N terminus, an adjacent region (the transcriptional activation domain) containing about 80 amino acids was found to be essential for transcriptional activation. Deletion of a region spanning about 175 amino acids of the C-proximal portion increased transcriptional activation markedly, revealing that this domain normally represses activation. Differences between the transcriptional activation and repression functions of c-Myb and v-Myb are discussed in the light of these functional domains. Our results suggest that transcriptional activation may be involved in transformation by myb gene products.

Original languageEnglish
Pages (from-to)5758-5762
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number15
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • General

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