Dehydroascorbate reductase and glutathione reductase play an important role in scavenging hydrogen peroxide during natural and artificial dehydration of Jatropha curcas seeds

Samar A. Omar, Nabil I. Elsheery, Hazem M. Kalaji, Zeng Fu Xu, Song Song-Quan, Robert Carpentier, Choon Hwan Lee, Suleyman I. Allakhverdiev

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Changes in H2O2 and the main antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), in endospermic and embryonic tissues were studied in developing and artificially dried Jatropha curcas seeds. Immature seeds were desiccation-tolerant at 80 days after flowering, as they were able to germinate fully after artificial drying on silica gel had reduced their water content to 10-12% of fresh weight. In both endospermic and embryonic tissues, H2O2 level and, consequently, lipid peroxide content, decreased during seed development as well as after artificial dehydration of developing seeds. All examined antioxidant enzymes except DHAR showed a decrease in total activity in mature stages as compared with early stages. Expression analysis of SOD genes revealed that the decrease in total SOD activities was related to the decrease in Cu/Zn-SOD expression, while the continuous activity of SOD during maturation was related to an increase in Mn-SOD expression. Artificial drying resulted in increased SOD and DHAR activity, irrespective of the developmental stage. Our results revealed weak participation of CAT and APX in H2O2 scavenging, as well as no significant alterations in GR activities either during maturation or after artificial drying. Changes in SOD and GR isoenzyme patterns occurred during maturation-related drying, but not after artificial drying. These results highlight the role of ascorbate-glutathione cycle enzymes (DHAR and GR) in H2O2 scavenging during maturation or after artificial drying of developing J. curcas seeds.

Original languageEnglish
Pages (from-to)469-480
Number of pages12
JournalJournal of Plant Biology
Volume55
Issue number6
DOIs
Publication statusPublished - Dec 1 2012

Keywords

  • Jatropha curcas
  • dehydroascorbate reductase
  • glutathione reductase
  • hydrogen peroxide
  • scavenging

ASJC Scopus subject areas

  • Plant Science

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