Degradation pathway of plant complex-type n-glycans: Identification and characterization of a key α1,3-fucosidase from glycoside hydrolase family 29

Shun Kato, Megumi Hayashi, Mai Kitagawa, Hiroyuki Kajiura, Megumi Maeda, Yoshinobu Kimura, Kiyohiko Igarashi, Masahiro Kasahara, Takeshi Ishimizu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Plant complex-type N-glycans are characterized by the presence of α1,3-linked fucose towards the proximal N-acetylglucosamine residue and β1,2-linked xylose towards the β-mannose residue. These glycans are ultimately degraded by the activity of several glycoside hydrolases. However, the degradation pathway of plant complex-type N-glycans has not been entirely elucidated because the gene encoding α1,3-fucosidase, a glycoside hydrolase acting on plant complex-type N-glycans, has not yet been identified, and its substrate specificity remains to be determined. In the present study, we found that AtFUC1 (an Arabidopsis GH29 α-fucosidase) is an α1,3-fucosidase acting on plant complex-type N-glycans. This fucosidase has been known to act on α1,4-fucoside linkage in the Lewis A epitope of plant complex-type N-glycans. We found that this glycoside hydrolase specifically acted on GlcNAcβ1–4(Fucα1–3)GlcNAc, a degradation product of plant complex-type N-glycans, by sequential actions of vacuolar α-mannosi-dase, β1,2-xylosidase, and endo-β-mannosidase. The AtFUC1-deficient mutant showed no distinct phenotypic plant growth features; however, it accumulated GlcNAcβ1–4 (Fucα1–3)GlcNAc, a substrate of AtFUC1. These results showed that AtFUC1 is an α1,3-fucosidase acting on plant complex-type N-glycans and elucidated the degradation pathway of plant complex-type N-glycans.

Original languageEnglish
Pages (from-to)305-317
Number of pages13
JournalBiochemical Journal
Volume475
Issue number1
DOIs
Publication statusPublished - Jan 15 2018

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alpha-L-Fucosidase
Glycoside Hydrolases
Polysaccharides
Degradation
Mannosidases
Xylosidases
Gene encoding
Fucose
Acetylglucosamine
Xylose
Substrates
Mannose
Substrate Specificity
Arabidopsis
Epitopes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Degradation pathway of plant complex-type n-glycans : Identification and characterization of a key α1,3-fucosidase from glycoside hydrolase family 29. / Kato, Shun; Hayashi, Megumi; Kitagawa, Mai; Kajiura, Hiroyuki; Maeda, Megumi; Kimura, Yoshinobu; Igarashi, Kiyohiko; Kasahara, Masahiro; Ishimizu, Takeshi.

In: Biochemical Journal, Vol. 475, No. 1, 15.01.2018, p. 305-317.

Research output: Contribution to journalArticle

Kato, Shun ; Hayashi, Megumi ; Kitagawa, Mai ; Kajiura, Hiroyuki ; Maeda, Megumi ; Kimura, Yoshinobu ; Igarashi, Kiyohiko ; Kasahara, Masahiro ; Ishimizu, Takeshi. / Degradation pathway of plant complex-type n-glycans : Identification and characterization of a key α1,3-fucosidase from glycoside hydrolase family 29. In: Biochemical Journal. 2018 ; Vol. 475, No. 1. pp. 305-317.
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