Degradation of triphenyltin by a fluorescent pseudomonad

H. Inoue, O. Takimura, H. Fuse, K. Murakami, Kazuo Kamimura, Y. Yamaoka

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Triphenyltin (TPT)-degrading bacteria were screened by a simple technique using a post-column high-performance liquid chromatography using 3,3',4',7-tetrahydroxyflavone as a post-column reagent for determination of TPT and its metabolite, diphenyltin (DPT). An isolated strain, strain CNR15, was identified as Pseudomonas chlororaphis on the basis of its morphological and biochemical features. The incubation of strain CNR15 in a medium containing glycerol, succinate, and 130 μM TPT resulted in the rapid degradation of TPT and the accumulation of approximately 40 μM DPT as the only metabolite after 48 h. The culture supernatants of strain CNR15, grown with or without TPT, exhibited a TPT degradation activity, whereas the resting ceils were not capable of degrading TPT. TPT was stoichiometrically degraded to DPT by the solid-phase extract of the culture supernatant, and benzene was detected as another degradation product. We found that the TPT degradation was catalyzed by low-molecular-mass substances (approximately 1,000 Da) in the extract, termed the TPT-degrading factor. The other fluorescent pseudomonads, P. chlororaphis ATCC 9446, Pseudomonas fluorescens ATCC 13525, and Pseudomonas aeruginosa ATCC 15692, also showed TPT degradation activity similar to strain CNR15 in the solid-phase extracts of their culture supernatants. These results suggest that the extracellular low-molecular-mass substance that is universally produced by the fluorescent pseudomonad could function as a potent catalyst to cometabolite TPT in the environment.

Original languageEnglish
Pages (from-to)3492-3498
Number of pages7
JournalApplied and Environmental Microbiology
Volume66
Issue number8
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Pseudomonas
degradation
Pseudomonas chlororaphis
metabolite
extracts
molecular weight
metabolites
benzene
liquid chromatography
Pseudomonas fluorescens
succinic acid
catalysts
Pseudomonas aeruginosa
catalyst
incubation
glycerol
high performance liquid chromatography
bacterium
triphenyltin
bacteria

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Degradation of triphenyltin by a fluorescent pseudomonad. / Inoue, H.; Takimura, O.; Fuse, H.; Murakami, K.; Kamimura, Kazuo; Yamaoka, Y.

In: Applied and Environmental Microbiology, Vol. 66, No. 8, 2000, p. 3492-3498.

Research output: Contribution to journalArticle

Inoue, H. ; Takimura, O. ; Fuse, H. ; Murakami, K. ; Kamimura, Kazuo ; Yamaoka, Y. / Degradation of triphenyltin by a fluorescent pseudomonad. In: Applied and Environmental Microbiology. 2000 ; Vol. 66, No. 8. pp. 3492-3498.
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