Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells

Kazunori Watanabe, Ryu Miyagawa, Chie Tomikawa, Rie Mizuno, Akihisa Takahashi, Hiroyuki Hori, Kenichi Ijiri

Research output: Contribution to journalArticle

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Abstract

Stress response mechanisms that modulate the dynamics of tRNA degradation and accumulation from the cytoplasm to the nucleus have been studied in yeast, the rat hepatoma and human cells. In the current study, we investigated tRNA degradation and accumulation in HeLa cells under various forms of stress. We found that initiator tRNAMet (tRNA(iMet)) was specifically degraded under heat stress. Two exonucleases, Xrn1 and Xrn2, are involved in the degradation of tRNA(iMet) in the cytoplasm and the nucleus, respectively. In addition to degradation, we observed accumulation of tRNA(iMet) in the nucleus. We also found that the mammalian target of rapamycin (mTOR), which regulates tRNA trafficking in yeast, is partially phosphorylated at Ser2448 in the presence of rapamycin and/or during heat stress. Our results suggest phosphorylation of mTOR may correlate with accumulation of tRNA(iMet) in heat-treated HeLa cells.

Original languageEnglish
Pages (from-to)4671-4685
Number of pages15
JournalNucleic acids research.
Volume41
Issue number8
DOIs
Publication statusPublished - Apr 2013

Fingerprint

RNA, Transfer, Met
HeLa Cells
Transfer RNA
Hot Temperature
Sirolimus
Cytoplasm
Yeasts
Exonucleases
Hepatocellular Carcinoma
Phosphorylation

ASJC Scopus subject areas

  • Genetics

Cite this

Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells. / Watanabe, Kazunori; Miyagawa, Ryu; Tomikawa, Chie; Mizuno, Rie; Takahashi, Akihisa; Hori, Hiroyuki; Ijiri, Kenichi.

In: Nucleic acids research., Vol. 41, No. 8, 04.2013, p. 4671-4685.

Research output: Contribution to journalArticle

Watanabe, Kazunori ; Miyagawa, Ryu ; Tomikawa, Chie ; Mizuno, Rie ; Takahashi, Akihisa ; Hori, Hiroyuki ; Ijiri, Kenichi. / Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells. In: Nucleic acids research. 2013 ; Vol. 41, No. 8. pp. 4671-4685.
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