Degeneration of hrpZ gene in Pseudomonas syringae pv. tabaci to evade tobacco defence

An arms race between tobacco and its bacterial pathogen

Kazuhiko Tsunemi, Fumiko Taguchi, Mizuri Marutani, Megumi Watanabe-Sugimoto, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The HrpZ harpin of Pseudomonas syringae is known to induce a hypersensitive response (HR) in some plants. In P. syringae pv. tabaci (Pta), the harpin gene hrpZ has been spontaneously disrupted by an internal deletion in its open reading frame and a frame shift. The loss of the ability of the recombinant harpin polypeptide of Pta to induce HR despite the high sensitivity of tobacco plants to harpin led us to investigate the meaning of the disrupted hrpZ gene in the virulence of Pta 6605. The hrpZ gene from P. syringae pv. pisi was introduced into wild-type (WT) Pta. The hrpZ-complemented Pta secreted harpin into the culture medium, but failed to cause disease symptoms by both infiltration and spray inoculation. Inoculation with the hrpZ-complemented Pta induced defence responses in tobacco plants, whereas the defence responses of tobacco plants were not prominent on inoculation with WT Pta. These results indicate that an ancestor of Pta might have disrupted hrpZ by an internal deletion to evade plant defences and confer the ability to cause disease in tobacco plants.

Original languageEnglish
Pages (from-to)709-714
Number of pages6
JournalMolecular Plant Pathology
Volume12
Issue number7
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Pseudomonas syringae pv. tabaci
Pseudomonas syringae
arms race
tobacco
Tobacco
pathogen
gene
pathogens
inoculation
Genes
genes
disease by symptom
hypersensitive response
Pseudomonas syringae pv. pisi
plant defense
virulence
ancestry
spray
infiltration
signs and symptoms (plants)

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Soil Science
  • Molecular Biology

Cite this

Degeneration of hrpZ gene in Pseudomonas syringae pv. tabaci to evade tobacco defence : An arms race between tobacco and its bacterial pathogen. / Tsunemi, Kazuhiko; Taguchi, Fumiko; Marutani, Mizuri; Watanabe-Sugimoto, Megumi; Inagaki, Yoshishige; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki.

In: Molecular Plant Pathology, Vol. 12, No. 7, 09.2011, p. 709-714.

Research output: Contribution to journalArticle

Tsunemi, Kazuhiko ; Taguchi, Fumiko ; Marutani, Mizuri ; Watanabe-Sugimoto, Megumi ; Inagaki, Yoshishige ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Ichinose, Yuki. / Degeneration of hrpZ gene in Pseudomonas syringae pv. tabaci to evade tobacco defence : An arms race between tobacco and its bacterial pathogen. In: Molecular Plant Pathology. 2011 ; Vol. 12, No. 7. pp. 709-714.
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