Deformation of chlorin rings in the photosystem II crystal structure

Keisuke Saito, Yasufumi Umena, Keisuke Kawakami, Jian-Ren Shen, Nobuo Kamiya, Hiroshi Ishikita

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The crystal structure of Photosystem II (PSII) analyzed at a resolution of 1.9 Å revealed deformations of chlorin rings in the chlorophylls for the first time. We investigated the degrees of chlorin ring deformation and factors that contributed to them in the PSII crystal structure, using a normal-coordinate structural decomposition procedure. The out-of-plane distortion of the PD1 chlorin ring can be described predominantly by a large "doming mode" arising from the axial ligand, D1-His198, as well as the chlorophyll side chains and PSII protein environment. In contrast, the deformation of PD2 was caused by a "saddling mode" arising from the D2-Trp191 ring and the doming mode arising from D2-His197. Large ruffling modes, which were reported to lower the redox potential in heme proteins, were observed in PD1 and ChlD1, but not in PD2 and ChlD2. Furthermore, as PD1 possessed the largest doming mode among the reaction center chlorophylls, the corresponding bacteriochlorophyll PL possessed the largest doming mode in bacterial photosynthetic reaction centers. However, the majority of the redox potential shift in the protein environment was determined by the electrostatic environment. The difference in the chlorin ring deformation appears to directly refer to the difference in "the local steric protein environment" rather than the redox potential value in PSII.

Original languageEnglish
Pages (from-to)4290-4299
Number of pages10
JournalBiochemistry
Volume51
Issue number21
DOIs
Publication statusPublished - May 29 2012

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Photosystem II Protein Complex
Chlorophyll
Crystal structure
Oxidation-Reduction
Photosynthetic Reaction Center Complex Proteins
Bacteriochlorophylls
Hemeproteins
Proteins
Static Electricity
Electrostatics
Ligands
Decomposition
chlorin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Deformation of chlorin rings in the photosystem II crystal structure. / Saito, Keisuke; Umena, Yasufumi; Kawakami, Keisuke; Shen, Jian-Ren; Kamiya, Nobuo; Ishikita, Hiroshi.

In: Biochemistry, Vol. 51, No. 21, 29.05.2012, p. 4290-4299.

Research output: Contribution to journalArticle

Saito, K, Umena, Y, Kawakami, K, Shen, J-R, Kamiya, N & Ishikita, H 2012, 'Deformation of chlorin rings in the photosystem II crystal structure', Biochemistry, vol. 51, no. 21, pp. 4290-4299. https://doi.org/10.1021/bi300428s
Saito, Keisuke ; Umena, Yasufumi ; Kawakami, Keisuke ; Shen, Jian-Ren ; Kamiya, Nobuo ; Ishikita, Hiroshi. / Deformation of chlorin rings in the photosystem II crystal structure. In: Biochemistry. 2012 ; Vol. 51, No. 21. pp. 4290-4299.
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