Deficiency screening for genomic regions with effects on environmental sensitivity of the sensory bristles of drosophila melanogaster

Kazuo Takahashi, Yasukazu Okada, Kouhei Teramura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Environmental canalization is defined as a reduction in the effect of external environmental perturbations on a phenotype, while phenotypic plasticity is defined as the production of different phenotypes in alternative environments. These terms describe different aspects of the same phenomenon, that is, the sensitivity of the phenotype to the environment. Genetic regulation of the environmental sensitivity has been a central topic in the field of evolutionary biology. In this study, we performed deficiency screening to detect genomic regions with effects on the environmental sensitivity of Drosophila melanogaster sensory bristles. We used a collection of isogenic deficiency strains established by the DrosDel Project for screening. We screened 423 genomic deficiencies that encompassed approximately 63.6% of the entire D. melanogaster genome. We identified 29 genomic deficiencies showing significant effects on environmental sensitivity, suggesting that multiple genomic regions may influence phenotypic variation. We also found significant correlations among the effects of deficiencies on environmental sensitivity for different bristle traits, suggesting that the same genetic mechanism can regulate environmental sensitivity of multiple traits. Current high-resolution mapping will facilitate the examination of individual candidate genes using mutations or RNAi approaches in future studies.

Original languageEnglish
Pages (from-to)2878-2890
Number of pages13
JournalEvolution
Volume66
Issue number9
DOIs
Publication statusPublished - 2012

Fingerprint

setae (animal)
Drosophila melanogaster
genomics
screening
Phenotype
phenotype
RNA Interference
environmental law
evolutionary biology
phenotypic plasticity
phenotypic variation
Genome
mutation
Mutation
genome
perturbation
Biological Sciences
Genes
effect
gene

Keywords

  • Developmental stability
  • Environmental sensitivity
  • Fluctuating asymmetry
  • Isogenic deficiency strains
  • Reaction norm

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Agricultural and Biological Sciences(all)

Cite this

Deficiency screening for genomic regions with effects on environmental sensitivity of the sensory bristles of drosophila melanogaster. / Takahashi, Kazuo; Okada, Yasukazu; Teramura, Kouhei.

In: Evolution, Vol. 66, No. 9, 2012, p. 2878-2890.

Research output: Contribution to journalArticle

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