Deficiency of PAR-2 gene increases acute focal ischemic brain injury

Guang Jin, Takeshi Hayashi, Junichi Kawagoe, Toshiaki Takizawa, Tetsuya Nagata, Isao Nagano, Mikio Syoji, Koji Abe

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The expression profile of the protease-activated receptor-2 (PAR-2) and effects of PAR-2 gene knockout (PAR-2 KO) on the infarct size were investigated after 60 minutes of transient middle cerebral artery occlusion (tMCAO) in mice in relation to phosphorylated extracellular signal-regulated kinase (p-ERK) and astrocyte activation. PAR-2 was normally distributed mainly in neurons of the central nervous system (CNS), and strongly upregulated at 8-24 hours after tMCAO. Deficiency of PAR-2 gene significantly increased the infarct volume and the number of TUNEL-positive cells at 24 hours of reperfusion. The strong neuronal expression of p-ERK was induced at 5 minutes as a peak after reperfusion in wild-type mice, but the signal change was significantly reduced in PAR-2 KO mice. Astroglial activation was also greatly inhibited at 24 hours after tMCAO in PAR-2 KO mice. These results show that the deficiency of PAR-2 gene increases the acute ischemic cerebral injury associating with suppression of neuronal ERK activation and reactive astroglial activation.

Original languageEnglish
Pages (from-to)302-313
Number of pages12
JournalJournal of Cerebral Blood Flow and Metabolism
Volume25
Issue number3
DOIs
Publication statusPublished - Mar 2005

Fingerprint

PAR-2 Receptor
Brain Injuries
Middle Cerebral Artery Infarction
Genes
Extracellular Signal-Regulated MAP Kinases
Reperfusion
Gene Knockout Techniques
In Situ Nick-End Labeling
Astrocytes
Central Nervous System
Neurons
Wounds and Injuries

Keywords

  • ERK
  • GFAP
  • Ischemia
  • Knockout mice
  • NeuN
  • PAR-2

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Deficiency of PAR-2 gene increases acute focal ischemic brain injury. / Jin, Guang; Hayashi, Takeshi; Kawagoe, Junichi; Takizawa, Toshiaki; Nagata, Tetsuya; Nagano, Isao; Syoji, Mikio; Abe, Koji.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 25, No. 3, 03.2005, p. 302-313.

Research output: Contribution to journalArticle

Jin, G, Hayashi, T, Kawagoe, J, Takizawa, T, Nagata, T, Nagano, I, Syoji, M & Abe, K 2005, 'Deficiency of PAR-2 gene increases acute focal ischemic brain injury', Journal of Cerebral Blood Flow and Metabolism, vol. 25, no. 3, pp. 302-313. https://doi.org/10.1038/sj.jcbfm.9600021
Jin, Guang ; Hayashi, Takeshi ; Kawagoe, Junichi ; Takizawa, Toshiaki ; Nagata, Tetsuya ; Nagano, Isao ; Syoji, Mikio ; Abe, Koji. / Deficiency of PAR-2 gene increases acute focal ischemic brain injury. In: Journal of Cerebral Blood Flow and Metabolism. 2005 ; Vol. 25, No. 3. pp. 302-313.
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