Deficiency of caspase-3 in MCF7 cells blocks Bax-mediated nuclear fragmentation but not cell death

Shunsuke Kagawa, J. Gu, T. Honda, T. J. McDonnell, S. G. Swisher, J. A. Roth, B. Fang

Research output: Contribution to journalArticle

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Abstract

Caspase-3 plays a critical role in a proteolytic cascade within the apoptosis signal pathway; this enzyme is commonly activated by numerous death signals and cleaves a variety of important cellular proteins. Using caspase-3-deficient MCF7 cells and clones stably transfected with the caspase-3 gene (MCF7/Casp3), we evaluated the role of caspase-3 in Bax-induced apoptosis. Bax overexpression induced cell death in both parental MCF7 cells and MCF7/Casp3 cells. The introduction of the caspase-3 gene did not change the rate of cell death. Caspase-3-deficient parental MCF7 cells, however, failed to undergo morphological nuclear and DNA fragmentation, whereas MCF7/casp3 cells displayed intact nuclear dismantling and DNA fragmentation. Caspase-3 deficiency, however, did not affect Bax-induced levels of poly(ADP-ribose) polymerase cleavage, caspase-6 activation, and lamin B cleavage. Together, these results suggest that a deficit in caspase-3 is not sufficient to block Bax-induced cell death.

Original languageEnglish
Pages (from-to)1474-1480
Number of pages7
JournalClinical Cancer Research
Volume7
Issue number5
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

MCF-7 Cells
Caspase 3
Cell Death
DNA Fragmentation
Lamin Type B
Caspase 6
Apoptosis
Poly(ADP-ribose) Polymerases
Genes
Signal Transduction
Clone Cells
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kagawa, S., Gu, J., Honda, T., McDonnell, T. J., Swisher, S. G., Roth, J. A., & Fang, B. (2001). Deficiency of caspase-3 in MCF7 cells blocks Bax-mediated nuclear fragmentation but not cell death. Clinical Cancer Research, 7(5), 1474-1480.

Deficiency of caspase-3 in MCF7 cells blocks Bax-mediated nuclear fragmentation but not cell death. / Kagawa, Shunsuke; Gu, J.; Honda, T.; McDonnell, T. J.; Swisher, S. G.; Roth, J. A.; Fang, B.

In: Clinical Cancer Research, Vol. 7, No. 5, 2001, p. 1474-1480.

Research output: Contribution to journalArticle

Kagawa, S, Gu, J, Honda, T, McDonnell, TJ, Swisher, SG, Roth, JA & Fang, B 2001, 'Deficiency of caspase-3 in MCF7 cells blocks Bax-mediated nuclear fragmentation but not cell death', Clinical Cancer Research, vol. 7, no. 5, pp. 1474-1480.
Kagawa, Shunsuke ; Gu, J. ; Honda, T. ; McDonnell, T. J. ; Swisher, S. G. ; Roth, J. A. ; Fang, B. / Deficiency of caspase-3 in MCF7 cells blocks Bax-mediated nuclear fragmentation but not cell death. In: Clinical Cancer Research. 2001 ; Vol. 7, No. 5. pp. 1474-1480.
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