Abstract
The Linc-Kit Sca-1 cell population in the bone marrow (BM) serves as the direct precursor for differentiation of myeloid cells. In this study, we report that deficiency in Fpr2, a G protein– coupled chemoattractant receptor in mice, is associated with reduced BM nucleated cells, including CD31Ly6C (granulocytes and monocytes), CD31/Ly6Cint (granuloid cells), and CD31/Ly6Chigh (predominantly monocytes) cells. In particular, the number of Linc-KitSca-1 (LKS) cells was reduced in Fpr2/ mouse BM. This was supported by observations of the reduced incorporation of intraperitoneally injected bromodeoxyuridine by cells in the c-Kit population from Fpr2/ mouse BM. Purified c-Kit cells from Fpr2/ mice showed reduced expansion when cultured in vitro with stem cell factor (SCF). SCF/c-Kit-mediated phosphorylation of P38, STAT1, Akt (Thr-308), and Akt (Ser-473) was also significantly reduced in c-Kit cells from Fpr2/ mice. Furthermore, Fpr2 agonists enhanced SCF-induced proliferation of c-Kit cells. Colony-forming unit assays revealed that CFU– granulocyte–macrophage formation of BM cells from Fpr2/ mice was significantly reduced. After heat-inactivated bacterial stimulation in the airway, the expansion of c-kit Sca-1 cells in BM and recruitment of Ly6G cells to the lungs and CD11b Ly6CTNF cells to the spleen of Fpr2/ mice was significantly reduced. These results demonstrate an important role for Fpr2 in the development of myeloid lineage precursors in mouse BM.
| Original language | English |
|---|---|
| Pages (from-to) | 13452-13463 |
| Number of pages | 12 |
| Journal | Journal of Biological Chemistry |
| Volume | 293 |
| Issue number | 35 |
| DOIs | |
| Publication status | Published - Jan 1 2018 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Deficiency in Fpr2 results in reduced numbers of LincKitSca1 myeloid progenitor cells. / Chen, Keqiang; Singh, Vijay K.; Tang, Peng; Bao, Zhiyao; He, Tianzhen; Xiang, Yi; Gong, Wanghua; Yoshimura, Teizo; Le, Yingying; Tessarollo, Lino; Chen, Xin; Wang, Ji Ming.
In: Journal of Biological Chemistry, Vol. 293, No. 35, 01.01.2018, p. 13452-13463.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Deficiency in Fpr2 results in reduced numbers of LincKitSca1 myeloid progenitor cells
AU - Chen, Keqiang
AU - Singh, Vijay K.
AU - Tang, Peng
AU - Bao, Zhiyao
AU - He, Tianzhen
AU - Xiang, Yi
AU - Gong, Wanghua
AU - Yoshimura, Teizo
AU - Le, Yingying
AU - Tessarollo, Lino
AU - Chen, Xin
AU - Wang, Ji Ming
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The Linc-Kit Sca-1 cell population in the bone marrow (BM) serves as the direct precursor for differentiation of myeloid cells. In this study, we report that deficiency in Fpr2, a G protein– coupled chemoattractant receptor in mice, is associated with reduced BM nucleated cells, including CD31Ly6C (granulocytes and monocytes), CD31/Ly6Cint (granuloid cells), and CD31/Ly6Chigh (predominantly monocytes) cells. In particular, the number of Linc-KitSca-1 (LKS) cells was reduced in Fpr2/ mouse BM. This was supported by observations of the reduced incorporation of intraperitoneally injected bromodeoxyuridine by cells in the c-Kit population from Fpr2/ mouse BM. Purified c-Kit cells from Fpr2/ mice showed reduced expansion when cultured in vitro with stem cell factor (SCF). SCF/c-Kit-mediated phosphorylation of P38, STAT1, Akt (Thr-308), and Akt (Ser-473) was also significantly reduced in c-Kit cells from Fpr2/ mice. Furthermore, Fpr2 agonists enhanced SCF-induced proliferation of c-Kit cells. Colony-forming unit assays revealed that CFU– granulocyte–macrophage formation of BM cells from Fpr2/ mice was significantly reduced. After heat-inactivated bacterial stimulation in the airway, the expansion of c-kit Sca-1 cells in BM and recruitment of Ly6G cells to the lungs and CD11b Ly6CTNF cells to the spleen of Fpr2/ mice was significantly reduced. These results demonstrate an important role for Fpr2 in the development of myeloid lineage precursors in mouse BM.
AB - The Linc-Kit Sca-1 cell population in the bone marrow (BM) serves as the direct precursor for differentiation of myeloid cells. In this study, we report that deficiency in Fpr2, a G protein– coupled chemoattractant receptor in mice, is associated with reduced BM nucleated cells, including CD31Ly6C (granulocytes and monocytes), CD31/Ly6Cint (granuloid cells), and CD31/Ly6Chigh (predominantly monocytes) cells. In particular, the number of Linc-KitSca-1 (LKS) cells was reduced in Fpr2/ mouse BM. This was supported by observations of the reduced incorporation of intraperitoneally injected bromodeoxyuridine by cells in the c-Kit population from Fpr2/ mouse BM. Purified c-Kit cells from Fpr2/ mice showed reduced expansion when cultured in vitro with stem cell factor (SCF). SCF/c-Kit-mediated phosphorylation of P38, STAT1, Akt (Thr-308), and Akt (Ser-473) was also significantly reduced in c-Kit cells from Fpr2/ mice. Furthermore, Fpr2 agonists enhanced SCF-induced proliferation of c-Kit cells. Colony-forming unit assays revealed that CFU– granulocyte–macrophage formation of BM cells from Fpr2/ mice was significantly reduced. After heat-inactivated bacterial stimulation in the airway, the expansion of c-kit Sca-1 cells in BM and recruitment of Ly6G cells to the lungs and CD11b Ly6CTNF cells to the spleen of Fpr2/ mice was significantly reduced. These results demonstrate an important role for Fpr2 in the development of myeloid lineage precursors in mouse BM.
UR - http://www.scopus.com/inward/record.url?scp=85052604502&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052604502&partnerID=8YFLogxK
U2 - 10.1074/jbc.RA118.002683
DO - 10.1074/jbc.RA118.002683
M3 - Article
C2 - 30018139
AN - SCOPUS:85052604502
VL - 293
SP - 13452
EP - 13463
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 35
ER -