Abstract
Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans.
| Original language | English |
|---|---|
| Pages (from-to) | 618-622 |
| Number of pages | 5 |
| Journal | Journal of Health Science |
| Volume | 56 |
| Issue number | 5 |
| Publication status | Published - Oct 2010 |
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Keywords
- Antimicrobial peptide
- Enterococcus faecalis
- Human α-defensin
- Innate immunity
ASJC Scopus subject areas
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis. / Miyoshi, Shin-ichi; Koyama, Kenta; Mizuno, Tamaki; Kashihara, Minoru; Maehara, Yoko; Nakao, Hiroshi.
In: Journal of Health Science, Vol. 56, No. 5, 10.2010, p. 618-622.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis
AU - Miyoshi, Shin-ichi
AU - Koyama, Kenta
AU - Mizuno, Tamaki
AU - Kashihara, Minoru
AU - Maehara, Yoko
AU - Nakao, Hiroshi
PY - 2010/10
Y1 - 2010/10
N2 - Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans.
AB - Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans.
KW - Antimicrobial peptide
KW - Enterococcus faecalis
KW - Human α-defensin
KW - Innate immunity
UR - http://www.scopus.com/inward/record.url?scp=78049273711&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78049273711&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:78049273711
VL - 56
SP - 618
EP - 622
JO - Journal of Health Science
JF - Journal of Health Science
SN - 1344-9702
IS - 5
ER -