Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis

Shin ichi Miyoshi, Kenta Koyama, Tamaki Mizuno, Minoru Kashihara, Yoko Maehara, Hiroshi Nakao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans.

Original languageEnglish
Pages (from-to)618-622
Number of pages5
JournalJournal of Health Science
Volume56
Issue number5
DOIs
Publication statusPublished - Oct 2010

Keywords

  • Antimicrobial peptide
  • Enterococcus faecalis
  • Human α-defensin
  • Innate immunity

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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