Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis

Shin ichi Miyoshi; Kenta Koyama; Tamaki Mizuno; Minoru Kashihara; Yoko Maehara; Hiroshi Nakao

doi:10.1248/jhs.56.618

Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis

Shin ichi Miyoshi, Kenta Koyama, Tamaki Mizuno, Minoru Kashihara, Yoko Maehara, Hiroshi Nakao

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans.

Original language	English
Pages (from-to)	618-622
Number of pages	5
Journal	Journal of Health Science
Volume	56
Issue number	5
DOIs	https://doi.org/10.1248/jhs.56.618
Publication status	Published - Oct 2010

Keywords

Antimicrobial peptide
Enterococcus faecalis
Human α-defensin
Innate immunity

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

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title = "Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis",

abstract = "Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans.",

keywords = "Antimicrobial peptide, Enterococcus faecalis, Human α-defensin, Innate immunity",

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AU - Miyoshi, Shin ichi

AU - Koyama, Kenta

AU - Mizuno, Tamaki

AU - Kashihara, Minoru

AU - Maehara, Yoko

AU - Nakao, Hiroshi

PY - 2010/10

Y1 - 2010/10

N2 - Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans.

AB - Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans.

KW - Antimicrobial peptide

KW - Enterococcus faecalis

KW - Human α-defensin

KW - Innate immunity

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Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis

Abstract

Keywords

ASJC Scopus subject areas

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