Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis

Shin-ichi Miyoshi, Kenta Koyama, Tamaki Mizuno, Minoru Kashihara, Yoko Maehara, Hiroshi Nakao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans.

Original languageEnglish
Pages (from-to)618-622
Number of pages5
JournalJournal of Health Science
Volume56
Issue number5
Publication statusPublished - Oct 2010

Fingerprint

Defensins
Enterococcus faecalis
Peptides
Cathelicidins
Antimicrobial Cationic Peptides
Poisons
Pathogens
Innate Immunity
Peptide Hydrolases
Anti-Bacterial Agents

Keywords

  • Antimicrobial peptide
  • Enterococcus faecalis
  • Human α-defensin
  • Innate immunity

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis. / Miyoshi, Shin-ichi; Koyama, Kenta; Mizuno, Tamaki; Kashihara, Minoru; Maehara, Yoko; Nakao, Hiroshi.

In: Journal of Health Science, Vol. 56, No. 5, 10.2010, p. 618-622.

Research output: Contribution to journalArticle

Miyoshi, Shin-ichi ; Koyama, Kenta ; Mizuno, Tamaki ; Kashihara, Minoru ; Maehara, Yoko ; Nakao, Hiroshi. / Defensive effects of human antimicrobial peptide α-defensins against Enterococcus faecalis. In: Journal of Health Science. 2010 ; Vol. 56, No. 5. pp. 618-622.
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