Defensin-I, an antimicrobial peptide present in the saliva of patients with oral diseases

Nobuyoshi Mizukawa, K. Sugiyama, T. Ueno, K. Mishima, S. Takagi, T. Sugahara

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Abstract

OBJECTIVES AND DESIGN: A preceding paper has noted a detection of defensin-I (HNP-I), a peptide with antimicrobial and cytotoxic properties, in the saliva of patients with oral squamous cell carcinoma. The present study deals with the presence of HNP-I in the saliva of patients with various oral diseases. METHODS: Whole saliva samples were obtained from the patients. HNP- I in the saliva was isolated and purified by HPLC and the amino acid sequence of the peptide was determined. The molecular weight of HNP-I was measured by mass spectrometry. The concentration of HNP-I in saliva was determined by comparing the height of eluted HNP-I with that of a synthetic HNP-I standard. RESULTS: The concentrations of HNP-I in the saliva of patients with oral lichen planus (n = 5), leukoplakia (n = 4), and glossitis associated with iron deficiency (n = 4) were 8.3 ± 4.3 μg ml-1, 13.2 ± 7.9 μg ml-1, and 11.4 ± 4.9 μg ml-1, (mean ± s.d.), respectively. These concentrations were significantly higher than those in healthy subjects (0.8 μg ml-1) (P <0.01). In contrast, salivary HNP-I concentrations in patients with glossodynia (n = 4) and oral discomfort (n = 4) were similar to those in healthy subjects. CONCLUSIONS: Since HNP-I is a non-specific defensive peptide present in neutrophils, it may play an important role in the protection against diseases such as oral lichen planus, leukoplakia, and glossitis associated with iron deficiency.

Original languageEnglish
Pages (from-to)139-142
Number of pages4
JournalOral Diseases
Volume5
Issue number2
Publication statusPublished - Apr 1999

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Keywords

  • Defensin
  • HNP-I
  • Oral disease
  • Reversed-phase HPLC
  • Saliva

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Mizukawa, N., Sugiyama, K., Ueno, T., Mishima, K., Takagi, S., & Sugahara, T. (1999). Defensin-I, an antimicrobial peptide present in the saliva of patients with oral diseases. Oral Diseases, 5(2), 139-142.