Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma

Nobuyoshi Mizukawa, K. Sugiyama, J. Fukunaga, T. Ueno, K. Mishima, S. Takagi, T. Sugahara

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The saliva of patients with oral squamous cell carcinoma was subjected to reversed-phase HPLC, and a peak (P 14) was eluted with a linear gradient of acetonitrile. The molecular weight of P14 was 3442 daltons by mass spectrometry. The results of amino acid sequencing by Edman degradation and homology studies indicated that P14 was consistent with a peptide, human α-defensin 1 (HNP-1) which belongs to the defensin family. The concentration of HNP-1 in the saliva of 6 squamous cell carcinoma patients before surgery and 11 after surgery was 12.3 ± 8.6 and 6.5 ± 5.9 μg/ml (mean ± S.D.), respectively. In contrast, minimal amounts of HNP-1 were found in the saliva of adenocarcinoma patients and healthy volunteers. The concentration of salivary HNP-1 correlated positively with serum squamous cell carcinoma related antigen (SCC-Ag) level (r = 0.879, p <0.01).

Original languageEnglish
Pages (from-to)4645-4649
Number of pages5
JournalAnticancer Research
Volume18
Issue number6 B
Publication statusPublished - 1998

Fingerprint

Defensins
Saliva
Squamous Cell Carcinoma
Peptides
Protein Sequence Analysis
Mass Spectrometry
Healthy Volunteers
Adenocarcinoma
Molecular Weight
High Pressure Liquid Chromatography
human neutrophil peptide 1
Serum

Keywords

  • Defensin-1
  • Reversed-phase HPLC
  • Saliva
  • SCC-Ag
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Mizukawa, N., Sugiyama, K., Fukunaga, J., Ueno, T., Mishima, K., Takagi, S., & Sugahara, T. (1998). Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma. Anticancer Research, 18(6 B), 4645-4649.

Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma. / Mizukawa, Nobuyoshi; Sugiyama, K.; Fukunaga, J.; Ueno, T.; Mishima, K.; Takagi, S.; Sugahara, T.

In: Anticancer Research, Vol. 18, No. 6 B, 1998, p. 4645-4649.

Research output: Contribution to journalArticle

Mizukawa, N, Sugiyama, K, Fukunaga, J, Ueno, T, Mishima, K, Takagi, S & Sugahara, T 1998, 'Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma', Anticancer Research, vol. 18, no. 6 B, pp. 4645-4649.
Mizukawa N, Sugiyama K, Fukunaga J, Ueno T, Mishima K, Takagi S et al. Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma. Anticancer Research. 1998;18(6 B):4645-4649.
Mizukawa, Nobuyoshi ; Sugiyama, K. ; Fukunaga, J. ; Ueno, T. ; Mishima, K. ; Takagi, S. ; Sugahara, T. / Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma. In: Anticancer Research. 1998 ; Vol. 18, No. 6 B. pp. 4645-4649.
@article{23bb7a8dd03f4911a50de0a2c763373c,
title = "Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma",
abstract = "The saliva of patients with oral squamous cell carcinoma was subjected to reversed-phase HPLC, and a peak (P 14) was eluted with a linear gradient of acetonitrile. The molecular weight of P14 was 3442 daltons by mass spectrometry. The results of amino acid sequencing by Edman degradation and homology studies indicated that P14 was consistent with a peptide, human α-defensin 1 (HNP-1) which belongs to the defensin family. The concentration of HNP-1 in the saliva of 6 squamous cell carcinoma patients before surgery and 11 after surgery was 12.3 ± 8.6 and 6.5 ± 5.9 μg/ml (mean ± S.D.), respectively. In contrast, minimal amounts of HNP-1 were found in the saliva of adenocarcinoma patients and healthy volunteers. The concentration of salivary HNP-1 correlated positively with serum squamous cell carcinoma related antigen (SCC-Ag) level (r = 0.879, p <0.01).",
keywords = "Defensin-1, Reversed-phase HPLC, Saliva, SCC-Ag, Squamous cell carcinoma",
author = "Nobuyoshi Mizukawa and K. Sugiyama and J. Fukunaga and T. Ueno and K. Mishima and S. Takagi and T. Sugahara",
year = "1998",
language = "English",
volume = "18",
pages = "4645--4649",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "6 B",

}

TY - JOUR

T1 - Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma

AU - Mizukawa, Nobuyoshi

AU - Sugiyama, K.

AU - Fukunaga, J.

AU - Ueno, T.

AU - Mishima, K.

AU - Takagi, S.

AU - Sugahara, T.

PY - 1998

Y1 - 1998

N2 - The saliva of patients with oral squamous cell carcinoma was subjected to reversed-phase HPLC, and a peak (P 14) was eluted with a linear gradient of acetonitrile. The molecular weight of P14 was 3442 daltons by mass spectrometry. The results of amino acid sequencing by Edman degradation and homology studies indicated that P14 was consistent with a peptide, human α-defensin 1 (HNP-1) which belongs to the defensin family. The concentration of HNP-1 in the saliva of 6 squamous cell carcinoma patients before surgery and 11 after surgery was 12.3 ± 8.6 and 6.5 ± 5.9 μg/ml (mean ± S.D.), respectively. In contrast, minimal amounts of HNP-1 were found in the saliva of adenocarcinoma patients and healthy volunteers. The concentration of salivary HNP-1 correlated positively with serum squamous cell carcinoma related antigen (SCC-Ag) level (r = 0.879, p <0.01).

AB - The saliva of patients with oral squamous cell carcinoma was subjected to reversed-phase HPLC, and a peak (P 14) was eluted with a linear gradient of acetonitrile. The molecular weight of P14 was 3442 daltons by mass spectrometry. The results of amino acid sequencing by Edman degradation and homology studies indicated that P14 was consistent with a peptide, human α-defensin 1 (HNP-1) which belongs to the defensin family. The concentration of HNP-1 in the saliva of 6 squamous cell carcinoma patients before surgery and 11 after surgery was 12.3 ± 8.6 and 6.5 ± 5.9 μg/ml (mean ± S.D.), respectively. In contrast, minimal amounts of HNP-1 were found in the saliva of adenocarcinoma patients and healthy volunteers. The concentration of salivary HNP-1 correlated positively with serum squamous cell carcinoma related antigen (SCC-Ag) level (r = 0.879, p <0.01).

KW - Defensin-1

KW - Reversed-phase HPLC

KW - Saliva

KW - SCC-Ag

KW - Squamous cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=0032407465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032407465&partnerID=8YFLogxK

M3 - Article

C2 - 9891534

AN - SCOPUS:0032407465

VL - 18

SP - 4645

EP - 4649

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 6 B

ER -