Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma

Nobuyoshi Mizukawa, K. Sugiyama, J. Fukunaga, T. Ueno, K. Mishima, S. Takagi, T. Sugahara

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Abstract

The saliva of patients with oral squamous cell carcinoma was subjected to reversed-phase HPLC, and a peak (P 14) was eluted with a linear gradient of acetonitrile. The molecular weight of P14 was 3442 daltons by mass spectrometry. The results of amino acid sequencing by Edman degradation and homology studies indicated that P14 was consistent with a peptide, human α-defensin 1 (HNP-1) which belongs to the defensin family. The concentration of HNP-1 in the saliva of 6 squamous cell carcinoma patients before surgery and 11 after surgery was 12.3 ± 8.6 and 6.5 ± 5.9 μg/ml (mean ± S.D.), respectively. In contrast, minimal amounts of HNP-1 were found in the saliva of adenocarcinoma patients and healthy volunteers. The concentration of salivary HNP-1 correlated positively with serum squamous cell carcinoma related antigen (SCC-Ag) level (r = 0.879, p <0.01).

Original languageEnglish
Pages (from-to)4645-4649
Number of pages5
JournalAnticancer Research
Volume18
Issue number6 B
Publication statusPublished - 1998

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Keywords

  • Defensin-1
  • Reversed-phase HPLC
  • Saliva
  • SCC-Ag
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Mizukawa, N., Sugiyama, K., Fukunaga, J., Ueno, T., Mishima, K., Takagi, S., & Sugahara, T. (1998). Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma. Anticancer Research, 18(6 B), 4645-4649.