Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1)

Yasuhiro Ishiga, Kasumi Takeuchi, Fumiko Taguchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Flagellin, an essential component of the bacterial flagellar filament, is capable of inducing a hypersensitive response (HR), including cell death, in a nonhost plant. A flagellin-defective mutant (ΔfliC) of Pseudomonas syringae pv. tabaci lacks both the flagellar filament and motility, whereas a flagellin-glycosylation-defective mutant (Δorf1) retains the flagellar filament but lacks the glycosyl modification of flagellin protein. To investigate the role of flagellin protein and its glycosylation in the interaction with its nonhost Arabidopsis thaliana, we analyzed plant responses after inoculation with these bacteria. Inoculation with wild-type P. syringae pv. tabaci induced HR, with the generation of reactive oxygen species and cell death. In contrast, inoculation with either ΔfliC or Δorf1 mutant induced a low level of HR, and inoculated leaves developed a disease-like yellowing. These mutant bacteria multiplied better than the wild-type bacteria in A. thaliana. These results indicate that A. thaliana expresses a defense reaction in response to the bacterial flagellin with its glycosyl structure.

Original languageEnglish
Pages (from-to)302-307
Number of pages6
JournalJournal of General Plant Pathology
Volume71
Issue number4
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Pseudomonas syringae pv. tabaci
flagellin
glycosylation
Arabidopsis thaliana
mutants
hypersensitive response
cell death
bacteria
plant response
reactive oxygen species
proteins

Keywords

  • Arabidopsis thaliana
  • Flagellin
  • Glycosylation
  • Hypersensitive reaction (HR)
  • Pseudomonas syringae

ASJC Scopus subject areas

  • Plant Science

Cite this

Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1). / Ishiga, Yasuhiro; Takeuchi, Kasumi; Taguchi, Fumiko; Inagaki, Yoshishige; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki.

In: Journal of General Plant Pathology, Vol. 71, No. 4, 08.2005, p. 302-307.

Research output: Contribution to journalArticle

@article{0f112aa597d5437c9fe4d6b494f8cf7a,
title = "Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1)",
abstract = "Flagellin, an essential component of the bacterial flagellar filament, is capable of inducing a hypersensitive response (HR), including cell death, in a nonhost plant. A flagellin-defective mutant (ΔfliC) of Pseudomonas syringae pv. tabaci lacks both the flagellar filament and motility, whereas a flagellin-glycosylation-defective mutant (Δorf1) retains the flagellar filament but lacks the glycosyl modification of flagellin protein. To investigate the role of flagellin protein and its glycosylation in the interaction with its nonhost Arabidopsis thaliana, we analyzed plant responses after inoculation with these bacteria. Inoculation with wild-type P. syringae pv. tabaci induced HR, with the generation of reactive oxygen species and cell death. In contrast, inoculation with either ΔfliC or Δorf1 mutant induced a low level of HR, and inoculated leaves developed a disease-like yellowing. These mutant bacteria multiplied better than the wild-type bacteria in A. thaliana. These results indicate that A. thaliana expresses a defense reaction in response to the bacterial flagellin with its glycosyl structure.",
keywords = "Arabidopsis thaliana, Flagellin, Glycosylation, Hypersensitive reaction (HR), Pseudomonas syringae",
author = "Yasuhiro Ishiga and Kasumi Takeuchi and Fumiko Taguchi and Yoshishige Inagaki and Kazuhiro Toyoda and Tomonori Shiraishi and Yuki Ichinose",
year = "2005",
month = "8",
doi = "10.1007/s10327-005-0201-8",
language = "English",
volume = "71",
pages = "302--307",
journal = "Journal of General Plant Pathology",
issn = "1345-2630",
publisher = "Springer Japan",
number = "4",

}

TY - JOUR

T1 - Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1)

AU - Ishiga, Yasuhiro

AU - Takeuchi, Kasumi

AU - Taguchi, Fumiko

AU - Inagaki, Yoshishige

AU - Toyoda, Kazuhiro

AU - Shiraishi, Tomonori

AU - Ichinose, Yuki

PY - 2005/8

Y1 - 2005/8

N2 - Flagellin, an essential component of the bacterial flagellar filament, is capable of inducing a hypersensitive response (HR), including cell death, in a nonhost plant. A flagellin-defective mutant (ΔfliC) of Pseudomonas syringae pv. tabaci lacks both the flagellar filament and motility, whereas a flagellin-glycosylation-defective mutant (Δorf1) retains the flagellar filament but lacks the glycosyl modification of flagellin protein. To investigate the role of flagellin protein and its glycosylation in the interaction with its nonhost Arabidopsis thaliana, we analyzed plant responses after inoculation with these bacteria. Inoculation with wild-type P. syringae pv. tabaci induced HR, with the generation of reactive oxygen species and cell death. In contrast, inoculation with either ΔfliC or Δorf1 mutant induced a low level of HR, and inoculated leaves developed a disease-like yellowing. These mutant bacteria multiplied better than the wild-type bacteria in A. thaliana. These results indicate that A. thaliana expresses a defense reaction in response to the bacterial flagellin with its glycosyl structure.

AB - Flagellin, an essential component of the bacterial flagellar filament, is capable of inducing a hypersensitive response (HR), including cell death, in a nonhost plant. A flagellin-defective mutant (ΔfliC) of Pseudomonas syringae pv. tabaci lacks both the flagellar filament and motility, whereas a flagellin-glycosylation-defective mutant (Δorf1) retains the flagellar filament but lacks the glycosyl modification of flagellin protein. To investigate the role of flagellin protein and its glycosylation in the interaction with its nonhost Arabidopsis thaliana, we analyzed plant responses after inoculation with these bacteria. Inoculation with wild-type P. syringae pv. tabaci induced HR, with the generation of reactive oxygen species and cell death. In contrast, inoculation with either ΔfliC or Δorf1 mutant induced a low level of HR, and inoculated leaves developed a disease-like yellowing. These mutant bacteria multiplied better than the wild-type bacteria in A. thaliana. These results indicate that A. thaliana expresses a defense reaction in response to the bacterial flagellin with its glycosyl structure.

KW - Arabidopsis thaliana

KW - Flagellin

KW - Glycosylation

KW - Hypersensitive reaction (HR)

KW - Pseudomonas syringae

UR - http://www.scopus.com/inward/record.url?scp=23944508248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23944508248&partnerID=8YFLogxK

U2 - 10.1007/s10327-005-0201-8

DO - 10.1007/s10327-005-0201-8

M3 - Article

AN - SCOPUS:23944508248

VL - 71

SP - 302

EP - 307

JO - Journal of General Plant Pathology

JF - Journal of General Plant Pathology

SN - 1345-2630

IS - 4

ER -